Anticariogenic Properties of ent-Pimarane Diterpenes Obtained by Microbial Transformation

Autor(es):Mostrar menos -

Severiano, Marcela E. ^[1] ; Simao, Marilia R. ^[1] ; Porto, Thiago S. ^[1] ; Martins, Carlos H. G. ^[1] ; Veneziani, Rodrigo C. S. ^[1] ; Furtado, Niege A. J. C. ^[2] ; Arakawa, Nilton S. ^[3] ; Said, Suraia ^[2] ; de Oliveira, Dioneia C. R. ^[3] ; Cunha, Wilson R. ^[1] ; Gregorio, Luiz E. ^[2] ; Ambrosio, Sergio R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Franca, Franca, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Vale do Paraiba Univ, Inst Res & Dev, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 15, n. 12, p. 8553-8566, 2010.
• Citações Web of Science: 16

Resumo

In the present work, the anticariogenic activities of three pimarane-type diterpenes obtained by fungal biotransformation were investigated. Among these metabolites, ent-8(14), 15-pimaradien-19-ol was the most active compound, displaying very promising MIC values (ranging from 1.5 to 4.0 mu g mL(-1)) against the main microorganisms responsible for dental caries: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis, and Lactobacillus casei. Time kill assays performed with ent-8(14), 15-pimaradien-19-ol against the primary causative agent S. mutans revealed that this compound only avoids growth of the inoculum in the first 12 h (bacteriostatic effect). However, its bactericidal effect is clearly noted thereafter (between 12 and 24 h). The curve profile obtained by combining ent-8(14), 15-pimaradien-19-ol and chlorhexidine revealed a significant reduction in the time necessary for killing S. mutans compared with each of these two chemicals alone. However, no synergistic effect was observed using the same combination in the checkerboard assays against this microorganism. In conclusion, our results point out that ent-8(14), 15-pimaradien-19-ol is an important metabolite in the search for new effective anticariogenic agents. (AU)

• Processo FAPESP: 07/54762-8 - Obtenção de análogos estruturais do ácido pimaradienóico através do processo de biotransformação fúngica e estudo do efeito destes metabólitos sobre a atividade da enzima TcDHODH e sobre a contração da musculatura lisa vascular de artéria aorta de ratos
• Beneficiário: Sergio Ricardo Ambrosio
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Jovens Pesquisadores
• Processo FAPESP: 08/53518-9 - Estudos sobre a biotransformacao fungica do acido pimaradienoico por glomella cingulata.
• Beneficiário: Marcela Etchebehere Severiano
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/55562-5 - Estudos sobre a biotransformação fúngica do ácido pimaradienoico por Mucor rouxii
• Beneficiário: Marilia Rodrigues Simão
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica