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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular determinants of caste differentiation in the highly eusocial honeybee Apis mellifera

Texto completo
Autor(es):
Barchuk, Angel R. ; Cristino, Alexandre S. ; Kucharski, Robert ; Costa, Luciano F. ; Simões, Zilá L. P. [5] ; Maleszka, Ryszard
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: BMC Developmental Biology; v. 7, n. 70 2007.
Área do conhecimento: Ciências Biológicas - Genética
Assunto(s):Abelhas   Apis mellifica
Resumo

In honeybees, differential feeding of female larvae promotes the occurrence of two different phenotypes, a queen and a worker, from identical genotypes, through incremental alterations, which affect general growth, and character state alterations that result in the presence or absence of specific structures. Although previous studies revealed a link between incremental alterations and differential expression of physiometabolic genes, the molecular changes accompanying character state alterations remain unknown. By using cDNA microarray analyses of > 6,000 Apis mellifera ESTs, we found 240 differentially expressed genes (DEGs) between developing queens and workers. Many genes recorded as up-regulated in prospective workers appear to be unique to A. mellifera, suggesting that the workers' developmental pathway involves the participation of novel genes. Workers up-regulate more developmental genes than queens, whereas queens up-regulate a greater proportion of physiometabolic genes, including genes coding for metabolic enzymes and genes whose products are known to regulate the rate of mass-transforming processes and the general growth of the organism ( e. g., tor). Many DEGs are likely to be involved in processes favoring the development of caste-biased structures, like brain, legs and ovaries, as well as genes that code for cytoskeleton constituents. Treatment of developing worker larvae with juvenile hormone (JH) revealed 52 JH responsive genes, specifically during the critical period of caste development. Using Gibbs sampling and Expectation Maximization algorithms, we discovered eight overrepresented cis-elements from four gene groups. Graph theory and complex networks concepts were adopted to attain powerful graphical representations of the interrelation between cis-elements and genes and objectively quantify the degree of relationship between these entities. We suggest that clusters of functionally related DEGs are co-regulated during caste development in honeybees. This network of interactions is activated by nutrition-driven stimuli in early larval stages. Our data are consistent with the hypothesis that JH is a key component of the developmental determination of queen-like characters. Finally, we propose a conceptual model of caste differentiation in A. mellifera based on gene-regulatory networks. (AU)

Processo FAPESP: 05/03926-5 - Genômica funcional de Apis mellifera: busca de novos genes e redes funcionais no contexto do desenvolvimento, da diferenciação de castas e da reprodução
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 04/08928-3 - Desenvolvimento e aplicação de métodos computacionais de alto desempenho para análise de sistemas físicos e biológicos
Beneficiário:Luiz Nunes de Oliveira
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 05/53083-4 - Expressão gênica e diferenciação de castas em Apis mellifera
Beneficiário:Angel Roberto Barchuk
Linha de fomento: Bolsas no Exterior - Pesquisa
Processo FAPESP: 04/03408-1 - Expressão gênica e diferenciação de castas em Apis mellifera
Beneficiário:Angel Roberto Barchuk
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 99/00719-6 - Abelhas africanizadas: análise integrada do processo de africanização de Apis mellifera com foco sobre determinantes da fertilidade de zangões, rainhas e operárias
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 05/00587-5 - Modelagem por redes (grafos) e técnicas de reconhecimento de padrões: estrutura, dinâmica e aplicações
Beneficiário:Roberto Marcondes Cesar Junior
Linha de fomento: Auxílio à Pesquisa - Temático