Hollow-fiber liquid-phase microextraction of amphetamine-type stimulants in human hair samples

Autor(es)

Pantaleao, Lorena do Nascimento ^[1] ; Passos Bismara Paranhos, Beatriz Aparecida ^[1] ; Yonamine, Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatography A; v. 1254, p. 1-7, SEP 7 2012.
• Citações Web of Science: 26

Resumo

A fast method was optimized and validated in order to quantify amphetamine-type stimulants (amphetamine, AMP; methamphetamine, MAMP; fenproporex, FPX; 3,4-methylenedioxymethamphetamine, MDMA; and 3,4-methylenedioxyamphetamine, MDA) in human hair samples. The method was based in an initial procedure of decontamination of hair samples (50 mg) with dichloromethane, followed by alkaline hydrolysis and extraction of the amphetamines using hollow-fiber liquid-phase micro extraction (HF-LPME) in the three-phase mode. Gas chromatography-mass spectrometry (GC-MS) was used for identification and quantification of the analytes. The LoQs obtained for all amphetamines (around 0.05 ng/mg) were below the cut-off value (0.2 ng/mg) established by the Society of Hair Testing (SoHT). The method showed to be simple and precise. The intra-day and inter-day precisions were within 10.6% and 11.4%, respectively, with the use of only two deuteratecl internal standards (AMP-d5 and MDMA-d5). By using the weighted least squares linear regression (1/x(2)), the accuracy of the method was satisfied in the lower concentration levels (accuracy values better than 87%). Hair samples collected from six volunteers who reported regular use of amphetamines were submitted to the developed method. Drug detection was observed in all samples of the volunteers. (c) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/03342-1 - Análise toxicológica simultânea de anfetaminas e benzodiazepínicos em amostras de cabelo
• Beneficiário: Lorena Do Nascimento Pantaleão
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/10317-3 - Análise toxicológica simultânea de anfetaminas e benzodiazepínicos em amostras de cabelo
• Beneficiário: Mauricio Yonamine
• Linha de fomento: Auxílio à Pesquisa - Regular