Occurrence and phylogenetic characterization of a baculovirus isolated from Culex quinquefasciatus in So Paulo State, Brazil

Autor(es)

da Cunha de Araujo Coutinho, Carlos Jose Pereira ^[1] ; Alves, Rafael ^[1] ; Sanscrainte, Neil D. ; Pinto Viviani, Andrea de Barros ^[2] ; dos Santos, Paulo Frugoli ^[2] ; Vasconcelos-Medeiros de Souza, Polyana A. ^[1] ; Vicente Guedes de Carvalho-Mello, Isabel Maria ^[1] ; Becnel, James J.

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil

^[2] Superintendencia Controle Endemias, BR-11665070 Caraguatatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 157, n. 9, p. 1741-1745, SEP 2012.
• Citações Web of Science: 3

Resumo

The aim of this study was to assess the occurrence of baculovirus infections in mosquitoes and characterize them by using molecular tools. Fortnightly collections were made of mosquito larvae in the city of Caraguatatuba. Six larvae of Culex quinquefasciatus were isolated that had white cysts (nodules) in epithelia cells of the posterior midgut, indicative of infection by a baculovirus. These larvae were subjected to DNA extraction. DNA was amplified, producing a fragment of around 600 nt of the lef-8 gene and 400 nt of Pif-2 gene. The sequences were aligned, using ClustalX 2.0, with partial sequences of lef-8 genes of baculoviruses isolated from members of other insect orders taken from the GenBank database and edited, and phylogenetic analysis was performed. Phylogenetic analysis performed with the lef-8 and pif-2 genes demonstrated that the baculovirus identified in Culex quinquefasciatus in the Caraguatatuba region is most closely related to the deltabaculovirus Culex nigripalpus nucleopolyhedrovirus. (AU)

• Processo FAPESP: 08/05700-2 - Microrganismos Entomopatogênicos para o Manejo Integrado de Dípteros Vetores: Caracterização Molecular, Patologia e Epidemiologia
• Beneficiário: Carlos José Pereira da Cunha de Araújo Coutinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular