Electroacupuncture reverses ethanol-induced locomotor sensitization and subsequent pERK expression in mice

Autor(es)

Fallopa, Paula ^[1] ; Escosteguy-Neto, Joao Carlos ^[1] ; Varela, Patricia ^[1] ; Carvalho, Thiago Nogueira ^[1] ; Tabosa, Angela M. F. ^[2] ; Santos-Junior, Jair Guilherme ^{[1, 2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Neurobiol Lab, Grp Neuronal Plast & Psychiat Disorders, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Orthoped & Traumatol, Div Chinese Med Acupuncture, Sao Paulo - Brazil

^[3] Fac Med Sci, Dept Physiol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY; v. 15, n. 8, p. 1121-1133, SEP 2012.
• Citações Web of Science: 6

Resumo

Extracellular signal-regulated kinase (ERK) plays a role in neuronal changes induced by repeated drug exposure. Given that electroacupuncture reverses locomotor sensitization induced by ethanol, we investigated whether this effect is parallel to ERK signalling. Mice received daily ethanol (2 g/kg i.p), for 21 d. Electroacupuncture was performed daily, during four (subsequent) days of ethanol withdrawal. The stimulus of 2 Hz or 100 Hz was provided in combinations of two acupoints: Ea1 (ST-36/Zusanli and PC-6/Neiguan) or Ea2 (Du-14/Dazhui and Du-20/Baihui). The specificity of acupoint effects were assessed by the inclusion of additional groups: Ea3 (ST-25/Tianshu - acupoint used for other non-related disorders), Sham1 or Sham2 (transdermic stimulation near the respective acupoints). The control group was only handled during withdrawal and the saline group was chronically treated with saline and handled similarly to controls. At day 5 of withdrawal, each group was divided in two subgroups, according to the presence or absence of ethanol challenge. The animals were perfused and their brains processed for pERK immunohistochemistry. Only Ea1 at 100 Hz (Ea1\_100) and Ea2 at 2 Hz (Ea2\_2) reversed locomotor sensitization induced by ethanol. Ethanol withdrawal decreases pERK in the dorsomedial striatum. This decrease is not abolished by electroacupuncture. Conversely, ethanol challenge increases pERK in the dorsomedial striatum, infralimbic cortex and central nucleus of amygdala. The specificity of acupoint stimulation to reverse these increases was seen only for Ea2\_2, in the infralimbic cortex and dorsomedial striatum. Therefore, behavioural effects of Ea2\_2 (but not Ea1\_100) depend, at least in part, on ERK signalling. (AU)

• Processo FAPESP: 07/55458-0 - Bases neuroquímicas dos efeitos da eletroacupuntura em camundongos submetidos a modelos animais de dependência ao etanol: sistema opióide, AMPc, PKA, pCREB e BDNF
• Beneficiário: Angela Maria Florencio Tabosa
• Linha de fomento: Auxílio à Pesquisa - Regular