Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Analysis of three Xanthomonas axonopodis pv. citri effector proteins in pathogenicity and their interactions with host plant proteins

Texto completo
Autor(es):
Dunger, German [1] ; Garofalo, Cecilia G. [1] ; Gottig, Natalia [1] ; Garavaglia, Betiana S. [1] ; Pereda Rosa, Maria C. [2] ; Farah, Chuck S. [2] ; Orellano, Elena G. [1] ; Ottado, Jorgelina [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Consejo Nacl Invest Cient & Tecn, Inst Biol Mol & Celular Rosario, RA-2000 Rosario, Santa Fe - Argentina
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: MOLECULAR PLANT PATHOLOGY; v. 13, n. 8, p. 865-876, OCT 2012.
Citações Web of Science: 13
Resumo

Xanthomonas axonopodis pv. citri, the bacterium responsible for citrus canker, uses effector proteins secreted by a type III protein secretion system to colonize its hosts. Among the putative effector proteins identified for this bacterium, we focused on the analysis of the roles of AvrXacE1, AvrXacE2 and Xac3090 in pathogenicity and their interactions with host plant proteins. Bacterial deletion mutants in avrXacE1, avrXacE2 and xac3090 were constructed and evaluated in pathogenicity assays. The avrXacE1 and avrXacE2 mutants presented lesions with larger necrotic areas relative to the wild-type strain when infiltrated in citrus leaves. Yeast two-hybrid studies were used to identify several plant proteins likely to interact with AvrXacE1, AvrXacE2 and Xac3090. We also assessed the localization of these effector proteins fused to green fluorescent protein in the plant cell, and observed that they co-localized to the subcellular spaces in which the plant proteins with which they interacted were predicted to be confined. Our results suggest that, although AvrXacE1 localizes to the plant cell nucleus, where it interacts with transcription factors and DNA-binding proteins, AvrXacE2 appears to be involved in lesion-stimulating disease 1-mediated cell death, and Xac3090 is directed to the chloroplast where its function remains to be clarified. (AU)

Processo FAPESP: 05/59243-3 - Análise estrutural e funcional de sistemas multiprotéticos para a patogenicidade de Xanthomonas axonopodis pv. citri
Beneficiário:Shaker Chuck Farah
Linha de fomento: Auxílio à Pesquisa - Temático