Determination of dimethyltryptamine and beta-carbolines (ayahuasca alkaloids) in plasma samples by LC-MS/MS

Autor(es)

Rodrigues Oliveira, Carolina Dizioli ^[1] ; Okai, Guilherme Goncalves ^[1] ; da Costa, Jose Luiz ^[2] ; de Almeida, Rafael Menck ^[1] ; Oliveira-Silva, Diogo ^[3] ; Yonamine, Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[2] Sao Paulo Criminalist Inst, Forens Toxicol & Chem Lab, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOANALYSIS; v. 4, n. 14, p. 1731-1738, JUL 2012.
• Citações Web of Science: 7

Resumo

Background: Ayahuasca is a psychoactive plant beverage originally used by indigenous people throughout the Amazon Basin, long before its modern use by syncretic religious groups established in Brazil, the USA and European countries. The objective of this study was to develop a method for quantification of dimethyltryptamine and beta-carbolines in human plasma samples. Results: The analytes were extracted by means of C18 cartridges and injected into LC-MS/MS, operated in positive ion mode and multiple reaction monitoring. The LOQs obtained for all analytes were below 0.5 ng/ml. By using the weighted least squares linear regression, the accuracy of the analytical method was improved at the lower end of the calibration curve (from 0.5 to 100 ng/ml; r(2)> 0.98). Conclusion: The method proved to be simple, rapid and useful to estimate administered doses for further pharmacological and toxicological investigations of ayahuasca exposure. (AU)

• Processo FAPESP: 06/00388-5 - Estudos de farmacocinetica e toxicologia do desenvolvimento dos alcaloides de ayahuasca.
• Beneficiário: Mauricio Yonamine
• Modalidade de apoio: Auxílio à Pesquisa - Regular