MAPK7 and MAP2K4 as prognostic markers in osteosarcoma

Autor(es)

Tesser-Gamba, Francine ^{[1, 2]} ; Petrilli, Antonio Sergio ^[1] ; de Seixas Alves, Maria Teresa ^{[1, 3]} ; Garcia Filho, Reynaldo Jesus ^[4] ; Juliano, Yara ^[5] ; Caminada Toledo, Silvia Regina ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Pediat Oncol Inst, Grp Apoio Ao Adolescente & Crianca Com Canc, Dept Pediat, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Pathol, BR-04023062 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Orthoped Surg & Traumatol, BR-04023062 Sao Paulo - Brazil

^[5] Univ Santo Amaro, Dept Publ Hlth, BR-04829300 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN PATHOLOGY; v. 43, n. 7, p. 994-1002, JUL 2012.
• Citações Web of Science: 18

Resumo

Osteosarcoma is a class of cancer originating from the bone, affecting mainly children and young adults. Cytogenetic studies showed the presence of rearrangements and recurrent gains in specific chromosomal regions, indicating the possible involvement of genes located in these regions during the pathogenesis of osteosarcoma. These studies investigated expression of 10 genes located in the chromosomal region involved in abnormalities in osteosarcoma, 1p36, 17p, and chromosome 19. The purpose of this study was to investigate the expression profile of genes located in regions involved in chromosomal rearrangements in osteosarcoma. We used quantitative real-time polymerase chain reaction to investigate the expression of 10 genes located in 1p36.3 (MTHFR, ERRFI1, FGR, E2F2), 17p (MAPK7, MAP2K4), and chromosome 19 (BBC3, FOSB, JUND, and BRAS), in 70 samples taken from 30 patients (30 prechemotherapy, 30 postchemotherapy, and 10 metastases specimens) and 10 healthy bones as a control sample. The most interesting results showed a strong association between the expression levels of MAPK7 and MAP2K4 genes and clinical parameters of osteosarcoma. Overexpression of these genes was significantly associated to a poor response to treatment (P = .0001 and P = .0049, respectively), tumor progression, and worse overall survival (P = .0052 and P = .0085, respectively), suggesting that MAPK7 and MAP2K4 could play an important role in osteosarcoma tumorigenesis. Thus, these genes could be good markers in assessing response to treatment and development of osteosarcoma. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/53869-3 - Investigação de eventos genéticos envolvidos no processo de tumorigênese do osteossarcoma
• Beneficiário: Silvia Regina Caminada de Toledo
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/02693-2 - Investigação da expressão de genes localizados em regiões de ganho cromossômico em osteossarcoma
• Beneficiário: Francine Tesser Gamba
• Linha de fomento: Bolsas no Brasil - Mestrado