CD25(+) T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses

Autor(es)

Ramos, Rodrigo Nalio ^[1] ; Oliveira, Carine Ervolino ^[1] ; Gasparoto, Thais Helena ^[1] ; de Souza Malaspina, Tatiana Salles ^[1] ; Belai, Eduardo Bertoli ^[1] ; Cavassani, Karen Angelica ^[2] ; Garlet, Gustavo Pompermaier ^[1] ; da Silva, Joao Santana ^[3] ; Campanelli, Ana Paula ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[2] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI - USA

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem & Immunol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Carcinogenesis; v. 33, n. 4, p. 902-909, APR 2012.
• Citações Web of Science: 10

Resumo

Squamous cell carcinoma (SCC) constitutes a microenvironment that could modulate the antitumor immune response. Also, tumor-infiltrating lymphocytes are believed to play complex regulatory roles in antitumor immunity against SCC. The presence of regulatory T cells (Tregs) has been associated with the suppression of tumor-reactive T cells. However, the underlying mechanism for this T cell dysfunction is not clear. We used a multistage model of SCC to examine the role of Treg cells during tumor development. 7,12-dimethylbenz{[}a]-anthracene/phorbol 12-myristate 13-acetate treatment and systemic depletion of Treg cells using an anti-CD25 monoclonal antibody (PC61) resulted in a decrease in the number and incidence of papilloma. Furthermore, CD25 depletion increased the proportion of CD8(+) and CD4(+) T cells that were isolated from tumor lesions. The levels of interleukin (IL)-1 beta, IL-10, IL-12, IL-13, interferon-gamma, transforming growth factor-beta and tumor necrosis factor-alpha, but not IL-17, were increased in the tumor microenvironment after Treg depletion. Therefore, our results indicated involvement of CD25(+) T cells in SCC development and in the suppression of the inflammatory immune response. (AU)

• Processo FAPESP: 08/10999-7 - Modulação da resposta imune durante o desenvolviemnto de carcinoma espinocelular
• Beneficiário: Ana Paula Campanelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/01617-8 - Estudo do papel de células T regulatórias no desenvolvimento de carcinoma oral de células escamosas
• Beneficiário: Rodrigo Nalio Ramos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/14127-7 - O papel do inflamassoma nos tumores quimicamente induzidos
• Beneficiário: Thaís Helena Gasparoto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/03471-9 - Modulação da resposta imune durante o desenvolvimento de carcinoma espinocelular
• Beneficiário: Eduardo Bertoli Belai
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/03859-0 - CD25 T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses
• Beneficiário: Ana Paula Campanelli
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo