CD25 T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses

• Processo: 12/03859-0
• Linha de fomento: Auxílio à Pesquisa - Publicações científicas - Artigo
• Vigência: 01 de abril de 2012 - 30 de setembro de 2012
• Área do conhecimento: Ciências Biológicas - Imunologia - Imunologia Celular
• Pesquisador responsável: Ana Paula Campanelli
• Beneficiário: Ana Paula Campanelli
• Instituição-sede: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brasil
• Assunto(s): Resposta imune Carcinoma de células escamosas Linfócitos T reguladores Citocinas Publicações de divulgação científica Artigo científico

Resumo

Squamous cell carcinoma (SCC) constitutes a microenvironment that could modulate the antitumor immune response. Also, tumor-infiltrating lymphocytes are believed to play complex regulatory roles in antitumor immunity against SCC. The presence ofregulatory T cells (Tregs) has been associated with the suppression of tumor-reactive T cells. However, the underlying mechanismfor this T cell dysfunction is not clear. We used a multistage model of SCC to examine the role of Treg cells during tumor development.7,12-dimethylbenz[a]-anthracene/phorbol 12-myristate 13-acetate treatment and systemic depletion of Treg cells using ananti-CD25 monoclonal antibody (PC61) resulted in a decrease in the number and incidence of papilloma. Furthermore, CD25 depletionincreased the proportion of CD81 and CD41 T cells that were isolated from tumor lesions. The levels of interleukin (IL)-1b, IL-10, IL-12, IL-13, interferon-g, transforming growth factorband tumor necrosis factor-a, but not IL-17, were increased in the tumor microenvironment after Treg depletion. Therefore, our results indicated involvement of CD251 T cells in SCC developmentand in the suppression of the inflammatory immune response. (AU)

Publicações científicas

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

RAMOS, RODRIGO NALIO; OLIVEIRA, CARINE ERVOLINO; GASPAROTO, THAIS HELENA; DE SOUZA MALASPINA, TATIANA SALLES; BELAI, EDUARDO BERTOLI; CAVASSANI, KAREN ANGELICA; GARLET, GUSTAVO POMPERMAIER; DA SILVA, JOAO SANTANA; CAMPANELLI, ANA PAULA. CD25(+) T cell depletion impairs murine squamous cell carcinoma development via modulation of antitumor immune responses. Carcinogenesis, v. 33, n. 4, p. 902-909, APR 2012. Citações Web of Science: 10.