MCF-7 Cells as a Three-Dimensional Model for the Study of Human Breast Cancer

Autor(es)

do Amaral, Jonatas Bussador ^[1] ; Rezende-Teixeira, Paula ^[1] ; Freitas, Vanessa Morais ^[1] ; Machado-Santelli, Glaucia Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Cell & Dev Biol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TISSUE ENGINEERING PART C-METHODS; v. 17, n. 11, p. 1097-1107, NOV 2011.
• Citações Web of Science: 21

Resumo

The increasing use of three-dimensional (3D) cell culture is because it reproduces in vitro results similar to in vivo results. Multicellular tumor spheroids generated in vitro exhibit important characteristics of avascular tumors, mainly with respect to tumor physiology and microenvironment. The interaction among cells in a tridimensional culture environment enhances cell differentiation and leads to luminal formation in some breast-derived cell cultures. The present work describes a method that permits luminal formation in breast adenocarcinoma cell (MCF-7)-derived spheroids in a 3D environment. In the proposed model, several relevant parameters, such as cell survival, apoptosis, autophagy, and E-cadherin expression, were analyzed to understand the organization of MCF-7 cells during different culture phases, including luminal and bud formation. (AU)

• Processo FAPESP: 06/01026-0 - Análise funcional de genes potencialmente regulados pelo receptor de estrógeno e/ou pelo oncogene ERBB2: implicações no diagnóstico, prognóstico e tratamento do câncer de mama
• Beneficiário: Maria Aparecida Nagai
• Modalidade de apoio: Auxílio à Pesquisa - Temático