Spectrophotometric flow injection monitoring of sulfide during sugar fermentation

Autor(es)

Silva, Claudineia R. ^[1] ; Barros, Valdemir A. F. ^[1] ; Basso, Luiz C. ^[1] ; Zagatto, Elias A. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ S Paulo, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 85, n. 3, p. 1703-1705, SEP 15 2011.
• Citações Web of Science: 4

Resumo

A spectrophotometric flow injection procedure involving N,N-dimethyl-p-phenylenediamine (DMPD) is applied to the sulfide monitoring of a sugar fermentation by Saccharomyces cerevisiae under laboratory conditions. The gaseous chemical species evolving from the fermentative process, mainly CO(2), are trapped allowing a cleaned sample aliquot to be collected and introduced into the flow injection analyzer. Measurement rate, signal repeatability, detection limit and reagent consumption per measurement were estimated as 150 h(-1) 0.36% (n=20), 0.014 mg L(-1) S and 120 mu g DMPD, respectively. The main characteristics of the monitoring record are discussed. The strategy is worthwhile for selecting yeast strain, increasing the industrial ethanol production and improving the quality of wines. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/00972-4 - Exploração de fluxos pulsados e difusão gasosa em sistema polivalente de análises em fluxo: monitoramento de sulfeto, sulfito e etanol durante a fermentação alcoólica.
• Beneficiário: Claudineia Rodrigues da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/07309-3 - Novas estratégias analíticas para estudos envolvendo biomoléculas
• Beneficiário: Elias Ayres Guidetti Zagatto
• Modalidade de apoio: Auxílio à Pesquisa - Temático