Isolation and characterization of 15 microsatellite loci in the stingless bee Plebeia remota (Apidae: Meliponini)

Autor(es)

Francisco, F. O. ^[1] ; Brito, R. M. ^[1] ; Santiago, L. R. ^[1] ; Goncalves, P. H. P. ^[1] ; Pioker, F. C. ^[2] ; Domingues-Yamada, A. M. T. ^[1] ; Arias, M. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Ecol, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS RESOURCES; v. 3, n. 3, p. 417-419, JUL 2011.
• Citações Web of Science: 15

Resumo

The destruction of Brazilian natural habitats has reduced bee populations and negative impacts of native flora pollination have been noticed. This work describes the isolation and characterization of microsatellite loci and evaluates them as molecular markers to study genetic variability of the stingless bee Plebeia remota. A microsatellite enriched genomic library was constructed and 15 primer pairs were designed for this species. The survey was conducted by analyzing 21 unrelated individuals. Genetic diversity indexes were calculated. The mean allelic richness was 6.3, the observed heterozygosity was 0.568, and the percentage of polymorphic loci was 93.33%. Also the primers were tested in cross-species amplification and showed promising results for P. droryana, P. emerina, P. lucii, P. meridionalis, P. pugnax, and P. saiqui. The microsatellite loci described here will be useful to evaluate genetic variability of stingless bees, and certainly will improve our knowledge about population dynamics especially in threatened environments. (AU)

• Processo FAPESP: 04/15801-0 - Biodiversidade e uso sustentável de polinizadores, com ênfase em abelhas Meliponini
• Beneficiário: Vera Lucia Imperatriz-Fonseca
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 10/50597-5 - Estudos populacionais e evolutivos em abelhas
• Beneficiário: Maria Cristina Arias
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/08546-4 - Estrutura genética de populações insulares e continentais de abelhas da Mata Atlântica
• Beneficiário: Flávio de Oliveira Francisco
• Linha de fomento: Bolsas no Brasil - Doutorado