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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and characterization of 15 microsatellite loci in the stingless bee Plebeia remota (Apidae: Meliponini)

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Author(s):
Francisco, F. O. [1] ; Brito, R. M. [1] ; Santiago, L. R. [1] ; Goncalves, P. H. P. [1] ; Pioker, F. C. [2] ; Domingues-Yamada, A. M. T. [1] ; Arias, M. C. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Ecol, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CONSERVATION GENETICS RESOURCES; v. 3, n. 3, p. 417-419, JUL 2011.
Web of Science Citations: 15
Abstract

The destruction of Brazilian natural habitats has reduced bee populations and negative impacts of native flora pollination have been noticed. This work describes the isolation and characterization of microsatellite loci and evaluates them as molecular markers to study genetic variability of the stingless bee Plebeia remota. A microsatellite enriched genomic library was constructed and 15 primer pairs were designed for this species. The survey was conducted by analyzing 21 unrelated individuals. Genetic diversity indexes were calculated. The mean allelic richness was 6.3, the observed heterozygosity was 0.568, and the percentage of polymorphic loci was 93.33%. Also the primers were tested in cross-species amplification and showed promising results for P. droryana, P. emerina, P. lucii, P. meridionalis, P. pugnax, and P. saiqui. The microsatellite loci described here will be useful to evaluate genetic variability of stingless bees, and certainly will improve our knowledge about population dynamics especially in threatened environments. (AU)

FAPESP's process: 04/15801-0 - Biodiversity and sustainable use of pollinators, with emphasis on Meliponini bees
Grantee:Vera Lucia Imperatriz-Fonseca
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 08/08546-4 - Genetic structure of island and mainland populations of bees from Atlantic Forest
Grantee:Flávio de Oliveira Francisco
Support Opportunities: Scholarships in Brazil - Doctorate