| Full text | |
| Author(s): |
Francisco, F. O.
[1]
;
Brito, R. M.
[1]
;
Santiago, L. R.
[1]
;
Goncalves, P. H. P.
[1]
;
Pioker, F. C.
[2]
;
Domingues-Yamada, A. M. T.
[1]
;
Arias, M. C.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Ecol, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | CONSERVATION GENETICS RESOURCES; v. 3, n. 3, p. 417-419, JUL 2011. |
| Web of Science Citations: | 15 |
| Abstract | |
The destruction of Brazilian natural habitats has reduced bee populations and negative impacts of native flora pollination have been noticed. This work describes the isolation and characterization of microsatellite loci and evaluates them as molecular markers to study genetic variability of the stingless bee Plebeia remota. A microsatellite enriched genomic library was constructed and 15 primer pairs were designed for this species. The survey was conducted by analyzing 21 unrelated individuals. Genetic diversity indexes were calculated. The mean allelic richness was 6.3, the observed heterozygosity was 0.568, and the percentage of polymorphic loci was 93.33%. Also the primers were tested in cross-species amplification and showed promising results for P. droryana, P. emerina, P. lucii, P. meridionalis, P. pugnax, and P. saiqui. The microsatellite loci described here will be useful to evaluate genetic variability of stingless bees, and certainly will improve our knowledge about population dynamics especially in threatened environments. (AU) | |
| FAPESP's process: | 10/50597-5 - Estudos populacionais e evolutivos em abelhas |
| Grantee: | Maria Cristina Arias |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 04/15801-0 - Biodiversity and sustainable use of pollinators, with emphasis on Meliponini bees |
| Grantee: | Vera Lucia Imperatriz-Fonseca |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 08/08546-4 - Genetic structure of island and mainland populations of bees from Atlantic Forest |
| Grantee: | Flávio de Oliveira Francisco |
| Support Opportunities: | Scholarships in Brazil - Doctorate |