Tolerogenic property of B-1b cells in a model of allergic reaction

Autor(es)

De Lorenzo, Beatriz H. P. ; Brito, Ronni R. N. ; Godoy, Luiz Claudio ; Lopes, José Daniel ^[4] ; Mariano, Mario

• Tipo de documento: Artigo Científico
• Fonte: Immunology Letters; v. 114, n. 2, p. 110-118, Dec. 2007.
• Área do conhecimento: Ciências Biológicas - Imunologia
• Assunto(s): Alergia e imunologia Células sanguíneas Linfócitos B

Resumo

Since B-1 cells were first described, their origin and function remain controversial. Given the ability to produce natural antibodies and large amounts of IL-10, there is a consensus about their role in innate immunity. More recently, however, B-1 cells have been associated to adaptive immunity as well, due to the demonstration of immunological memory and antigen presentation capability. Here we demonstrate that adoptive transfer of pre-sensitized B-1b cells (obtained from OVA-sensitized mice) to naïve B-1 deficient animals, drastically affects the ability of transplanted animals to mount an adaptive response upon immunization with OVA. In contrast to naïve B-1 populated mice, mice transplanted with sensitized B-1 exhibit lower anti-OVA antibody levels, milder footpad swelling in response to OVA subcutaneous injection and reduced granulomatous reaction to OVA-coated beads. Moreover, we show that these pre-sensitized B-1 cells, when acting as APCs, induce poor T cell proliferation in vitro when compared with macrophages or B-1 cells obtained from naïve mice. This property may be due in part to insufficient expression of the co-stimulatory molecule CD86, necessary for optimal antigen presentation. In conclusion, our data suggest a novel role for B-1 cells as part of suppressor mechanisms in the immune system. (AU)

• Processo FAPESP: 99/11144-4 - Participação da glicoproteína GP43 e de células b-1 na gênese do granuloma paracoccidioidomicótico
• Beneficiário: José Daniel Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Temático