Chloroplast microsatellite markers for the Neotropical orchid genus Epidendrum, and cross-amplification in other Laeliinae species (Orchidaceae)

Autor(es)

Pinheiro, Fabio ^[1] ; Palma-Silva, Clarisse ^[2] ; de Barros, Fabio ^[3] ; Felix, Leonardo P. ^[4] ; Lexer, Christian ^[5] ; Cozzolino, Salvatore ^[6] ; Fay, Mike F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bot, Inst Biociencias, BR-05508900 Sao Paulo - Brazil

^[2] Royal Bot Gardens Kew, Jodrell Lab, Richmond TW9 3DS, Surrey - England

^[3] Inst Bot Secao Orquidario Estado, BR-04301012 Sao Paulo - Brazil

^[4] Univ Fed Paraiba, Dept Fitotecnia, Lab Citogenet Vegetal, Areira, PB - Brazil

^[5] Univ Fribourg, Dept Biol, Unit Ecol & Evolut, CH-1700 Fribourg - Switzerland

^[6] Univ Naples Federico 2, Dipartimento Biol Strutturale & Funz, I-80100 Naples - Italy

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS RESOURCES; v. 1, n. 1, p. 505-511, DEC 2009.
• Citações Web of Science: 15

Resumo

One of the most significant challenges confronting orchid researchers is the lack of specific molecular markers, mainly for species in the Neotropics. Here we report the first set of specific chloroplast microsatellite primers (cpSSR) developed for Neotropical orchids. In total, nine polymorphic cpSSR loci were isolated and characterized in four species occurring in the Brazilian Atlantic Rainforest: Epidendrum cinnabarinum, E. denticulatum, E. fulgens and E. puniceoluteum. Levels of intraspecific polymorphism were characterized using two populations for each species, with 13-20 individuals each. Allele numbers varied from two to three per locus, while the number of haplotypes ranged from three to six per species. Extensive differentiation among the taxa was detected. All markers were successfully cross-amplified in eight other different genera. These cpSSRs markers will enable novel insights into the evolution of this important Neotropical genus. (AU)

• Processo FAPESP: 06/54189-3 - Implicações taxonômicas da estrutura genética de populações naturais de E. cinnabarinum Salzm., E. denticulatum Barb. Rodr. e E. fulgens Brongn.
• Beneficiário: Fábio de Barros
• Modalidade de apoio: Auxílio à Pesquisa - Regular