Electrochemical detection of carbamate pesticides in fruit and vegetables with a biosensor based on acetylcholinesterase immobilised on a composite of polyaniline-carbon nanotubes

Autor(es)

Cesarino, Ivana ^[1] ; Moraes, Fernando C. ^[1] ; Lanza, Marcos R. V. ^[1] ; Machado, Sergio A. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 135, n. 3, p. 873-879, DEC 1 2012.
• Citações Web of Science: 100

Resumo

A sensitive electrochemical acetylcholinesterase (AChE) biosensor was successfully developed on polyaniline (PANI) and multi-walled carbon nanotubes (MWCNTs) core-shell modified glassy carbon electrode (GC), and used to detect carbamate pesticides in fruit and vegetables (apple, broccoli and cabbage). The pesticide biosensors were applied in the detection of carbaryl and methomyl pesticides in food samples using chronoamperometry (CA). The GC/MWCNT/PANI/AChE biosensor exhibited detection limits of 1.4 and 0.95 mu mol L-1, respectively, for carbaryl and methomyl. These detection limits were below the allowable concentrations set by Brazilian regulation standards for the samples in which these pesticides were analysed. Reproducibility and repeatability values of 2.6% and 3.2%, respectively, were obtained in the conventional procedure. The proposed biosensor was successfully applied in the determination of carbamate pesticides in cabbage, broccoli and apple samples without any spiking procedure. The obtained results were in full agreement with those from the HPLC procedure. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/11049-2 - Desenvolvimento e aplicação de sensores eletroquímicos baseados em nanotubos de carbono alinhados para a detecção de interferentes endócrinos em amostras ambientais
• Beneficiário: Fernando Cruz de Moraes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/11567-3 - Análise in situ dos subprodutos formados na degradação eletroquímica de hidrocarbonetos monoaromáticos
• Beneficiário: Ivana Cesarino
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado