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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Electrochemical detection of carbamate pesticides in fruit and vegetables with a biosensor based on acetylcholinesterase immobilised on a composite of polyaniline-carbon nanotubes

Texto completo
Autor(es):
Cesarino, Ivana [1] ; Moraes, Fernando C. [1] ; Lanza, Marcos R. V. [1] ; Machado, Sergio A. S. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Food Chemistry; v. 135, n. 3, p. 873-879, DEC 1 2012.
Citações Web of Science: 100
Resumo

A sensitive electrochemical acetylcholinesterase (AChE) biosensor was successfully developed on polyaniline (PANI) and multi-walled carbon nanotubes (MWCNTs) core-shell modified glassy carbon electrode (GC), and used to detect carbamate pesticides in fruit and vegetables (apple, broccoli and cabbage). The pesticide biosensors were applied in the detection of carbaryl and methomyl pesticides in food samples using chronoamperometry (CA). The GC/MWCNT/PANI/AChE biosensor exhibited detection limits of 1.4 and 0.95 mu mol L-1, respectively, for carbaryl and methomyl. These detection limits were below the allowable concentrations set by Brazilian regulation standards for the samples in which these pesticides were analysed. Reproducibility and repeatability values of 2.6% and 3.2%, respectively, were obtained in the conventional procedure. The proposed biosensor was successfully applied in the determination of carbamate pesticides in cabbage, broccoli and apple samples without any spiking procedure. The obtained results were in full agreement with those from the HPLC procedure. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/11049-2 - Desenvolvimento e aplicação de sensores eletroquímicos baseados em nanotubos de carbono alinhados para a detecção de interferentes endócrinos em amostras ambientais
Beneficiário:Fernando Cruz de Moraes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/11567-3 - Análise in situ dos subprodutos formados na degradação eletroquímica de hidrocarbonetos monoaromáticos
Beneficiário:Ivana Cesarino
Linha de fomento: Bolsas no Brasil - Pós-Doutorado