From Amazonia to the Atlantic forest: Molecular phylogeny of Phyzelaphryninae frogs reveals unexpected diversity and a striking biogeographic pattern emphasizing conservation challenges

Autor(es)

Fouquet, Antoine ^{[1, 2]} ; Loebmann, Daniel ^[3] ; Castroviejo-Fisher, Santiago ^[4] ; Padial, Jose M. ^[4] ; Orrico, Victor G. D. ^[5] ; Lyra, Mariana L. ^[5] ; Roberto, Igor Joventino ^[6] ; Kok, Philippe J. R. ^{[7, 8]} ; Haddad, Celio F. B. ^[5] ; Rodrigues, Miguel T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508090 Sao Paulo - Brazil

^[2] CNRS Guyane USR 3456, Cayenne 97300 - French Guiana

^[3] Univ Fed Rio Grande, Inst Ciencias Biol, Lab Vertebrados Terr, BR-96203900 Rio Grande, RS - Brazil

^[4] Amer Museum Nat Hist, Dept Herpetol, New York, NY 10024 - USA

^[5] Univ Estadual Paulista, Inst Biociencias, Dept Zool, BR-13506900 Rio Claro, SP - Brazil

^[6] Univ Reg Cariri URCA, Zool Lab, Dept Ciencias Fis & Biol, BR-63105000 Crato, Ceara - Brazil

^[7] Royal Belgian Inst Nat Sci, Dept Vertebrates, B-1000 Brussels - Belgium

^[8] Vrije Univ Brussel, Dept Biol, Unit Ecol & Systemat, B-1050 Brussels - Belgium

• Tipo de documento: Artigo Científico
• Fonte: Molecular Phylogenetics and Evolution; v. 65, n. 2, p. 547-561, NOV 2012.
• Citações Web of Science: 64

Resumo

Documenting the Neotropical amphibian diversity has become a major challenge facing the threat of global climate change and the pace of environmental alteration. Recent molecular phylogenetic studies have revealed that the actual number of species in South American tropical forests is largely underestimated, but also that many lineages are millions of years old. The genera Phyzelaphryne (1 sp.) and Adelophryne (6 spp.), which compose the subfamily Phyzelaphryninae, include poorly documented, secretive, and minute frogs with an unusual distribution pattern that encompasses the biotic disjunction between Amazonia and the Atlantic forest. We generated >5.8 kb sequence data from six markers for all seven nominal species of the subfamily as well as for newly discovered populations in order to (1) test the monophyly of Phyzelaphryninae, Adelophryne and Phyzelaphryne, (2) estimate species diversity within the subfamily, and (3) investigate their historical biogeography and diversification. Phylogenetic reconstruction confirmed the monophyly of each group and revealed deep subdivisions within Adelophryne and Phyzelaphryne, with three major clades in Adelophryne located in northern Amazonia, northern Atlantic forest and southern Atlantic forest. Our results suggest that the actual number of species in Phyzelaphryninae is, at least, twice the currently recognized species diversity, with almost every geographically isolated population representing an anciently divergent candidate species. Such results highlight the challenges for conservation, especially in the northern Atlantic forest where it is still degraded at a fast pace. Molecular dating revealed that Phyzelaphryninae originated in Amazonia and dispersed during early Miocene to the Atlantic forest. The two Atlantic forest clades of Adelophryne started to diversify some 7 Ma minimum, while the northern Amazonian Adelophryne diversified much earlier, some 13 Ma minimum. This striking biogeographic pattern coincides with major events that have shaped the face of the South American continent, as we know it today. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 03/10335-8 - Sistemática e evolução da herpetofauna neotropical
• Beneficiário: Miguel Trefaut Urbano Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/57067-9 - Filogenia do grupo de dendropsophus marmoratus (anura:hylidae).
• Beneficiário: Victor Goyannes Dill Orrico
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50146-6 - Filogeografia comparada, filogenia, modelagem paleoclimática e taxonomia de répteis e anfíbios neotropicais
• Beneficiário: Miguel Trefaut Urbano Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 10/51071-7 - Species boundaries and evolutionary history of Leptodactylus marmoratus species group (Amphibia: Anura: Leptodactylidae): tracking big changes in Amazonia's past with small frogs and...
• Beneficiário: Miguel Trefaut Urbano Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/50124-0 - Utilização de marcadores moleculares para auxiliar no entendimento da diversidade de anfíbios neotropicais
• Beneficiário: Mariana Lúcio Lyra
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado