Photobiostimulation on Wound Healing Treatment by ClAlPc-nanoemulsion from a Multiple-Wavelength Portable Light Source on a 3D-Human Stem Cell Dermal Equivalent

Autor(es)

Primo, F. L. ^[1] ; de Paula, L. B. ^[1] ; de Siqueira-Moura, M. P. ^[1] ; Tedesco, A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Lab Fotobiol & Fotomed, Ctr Nanotecnol & Engn Teci, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Current Medicinal Chemistry; v. 19, n. 30, p. 5157-5163, OCT 2012.
• Citações Web of Science: 11

Resumo

This research evaluated the effect of multiple-wave lasertherapy on the healing process of surgical wounds based on in vitro models denominated stem-dermal equivalents. These human skin models were obtained from a co-culture of dermal cells and bone marrow mesenchymal stem cells. The experimental tests were carried out using a LED portable to multiple waves (operating at 660 nm and 810 nm) at different doses to induce photobiostimulation (10 to 70 mJ.cm(-2)). Moreover, a photosensitizer drug was employed as a new advanced designed nanomaterial, being a nanoemulsion with biopolymers to obtain an efficient drug delivery system to release lipophilic compounds. The studies were performed considering the light combination application monitoring the kinetic contraction of the dermal equivalent model and the quantification of important macromolecules (as metaloproteases derivatives), related directly with wound healing process. Results showed that an appropriate photomodulation using the combination of both wavelengths (in the red and infrared range) is possible, such that it can contribute to wound healing therapy and/or other pathological skin disease treatment. (AU)

• Processo FAPESP: 09/15363-6 - Desenvolvimento e caracterização de Equivalentes Dérmicos modificados com células tronco mesenquimais como modelos para avaliação de matriz extracelular após fotoestímulo com diodo-laser de baixa potência
• Beneficiário: Fernando Lucas Primo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático