Vascular endothelial growth factor A (VEGFA) modulates bovine placenta steroidogenesis in vitro

Autor(es)

Sousa, L. M. M. C. ^[1] ; Campos, D. B. ^[1] ; Fonseca, V. U. ^[1] ; Viau, P. ^[2] ; Kfoury, Jr., J. R. ^[1] ; Oliveira, C. A. ^[2] ; Binelli, M. ^[2] ; Buratini, Jr., J. ^[3] ; Papa, P. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sect Anat, BR-05508270 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil

^[3] Univ Sao Paulo State, Inst Biosci, Dept Physiol, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Placenta; v. 33, n. 10, p. 788-794, OCT 2012.
• Citações Web of Science: 7

Resumo

Our objectives were to investigate the possible role of VEGFA in bovine placenta steroid synthesis and to determine whether cloned derived placental cells present similar responses as non-cloned ones. Placental cells from cloned (term) and non-cloned (days 90, 150, 210 and term) pregnancies were isolated and treated with VEGFA (50 ng/ml) for 24, 48 or 96 h. Progesterone (P-4) and estrone sulfate (E1S) were assessed by RIA, while aromatase P450-positive cells were quantified using the point counting test. The percentages of steroidogenic and non-steroidogenic populations were determined by flow cytometry. VEGFA augmented or decreased P-4 and E1S concentrations as well as aromatase P450-positive cell density, depending on gestational age and time in culture. The percentage of steroidogenic cells was lower than that of non-steroidogenic ones for each culture time (P < 0.05). VEGFA treatment did not change the proportion of steroidogenic and non-steroidogenic cells. Placental cells derived from cloned pregnancies presented higher concentrations of E1S and P4 than the non-cloned group. However, aromatase P450-positive cells were similar between groups (P > 0.05). VEGFA treatment altered P-4 and E1S levels in placental cells depending on type of gestation. These results suggest that VEGFA acts locally in the bovine placenta to modulate steroidogenesis during gestation, but in a different pattern between cloned and non-cloned derived placental cells at term. Therefore, this factor can be considered an important regulator of placental development and function. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 02/07392-7 - Expressão do VEGF e bFGF na placenta bovina e seus efeitos sobre a produção de hormônios esteróides neste órgão
• Beneficiário: Paula de Carvalho Papa Keohane
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 05/51899-7 - Efeitos do vegf e bfgf sobre a producao de estrogenos na placenta de bovinos clonados e nao clonados.
• Beneficiário: Liza Margareth Medeiros de Carvalho Sousa
• Modalidade de apoio: Bolsas no Brasil - Mestrado