| Texto completo | |
| Autor(es): |
Sousa, L. M. M. C.
[1]
;
Campos, D. B.
[1]
;
Fonseca, V. U.
[1]
;
Viau, P.
[2]
;
Kfoury, Jr., J. R.
[1]
;
Oliveira, C. A.
[2]
;
Binelli, M.
[2]
;
Buratini, Jr., J.
[3]
;
Papa, P. C.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sect Anat, BR-05508270 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil
[3] Univ Sao Paulo State, Inst Biosci, Dept Physiol, Botucatu, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Placenta; v. 33, n. 10, p. 788-794, OCT 2012. |
| Citações Web of Science: | 7 |
| Resumo | |
Our objectives were to investigate the possible role of VEGFA in bovine placenta steroid synthesis and to determine whether cloned derived placental cells present similar responses as non-cloned ones. Placental cells from cloned (term) and non-cloned (days 90, 150, 210 and term) pregnancies were isolated and treated with VEGFA (50 ng/ml) for 24, 48 or 96 h. Progesterone (P-4) and estrone sulfate (E1S) were assessed by RIA, while aromatase P450-positive cells were quantified using the point counting test. The percentages of steroidogenic and non-steroidogenic populations were determined by flow cytometry. VEGFA augmented or decreased P-4 and E1S concentrations as well as aromatase P450-positive cell density, depending on gestational age and time in culture. The percentage of steroidogenic cells was lower than that of non-steroidogenic ones for each culture time (P < 0.05). VEGFA treatment did not change the proportion of steroidogenic and non-steroidogenic cells. Placental cells derived from cloned pregnancies presented higher concentrations of E1S and P4 than the non-cloned group. However, aromatase P450-positive cells were similar between groups (P > 0.05). VEGFA treatment altered P-4 and E1S levels in placental cells depending on type of gestation. These results suggest that VEGFA acts locally in the bovine placenta to modulate steroidogenesis during gestation, but in a different pattern between cloned and non-cloned derived placental cells at term. Therefore, this factor can be considered an important regulator of placental development and function. (C) 2012 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 02/07392-7 - Expressão do VEGF e bFGF na placenta bovina e seus efeitos sobre a produção de hormônios esteróides neste órgão |
| Beneficiário: | Paula de Carvalho Papa Keohane |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 05/51899-7 - Efeitos do vegf e bfgf sobre a producao de estrogenos na placenta de bovinos clonados e nao clonados. |
| Beneficiário: | Liza Margareth Medeiros de Carvalho Sousa |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |