FTIR and SEM analysis of CO2 laser irradiated human enamel

Autor(es)

Correa-Afonso, Alessandra Marques ^[1] ; Bachmann, Luciano ^[2] ; de Almeida, Cintia Guimaraes ^[1] ; Milori Corona, Silmara Aparecida ^[3] ; Borsatto, Maria Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Clin Infantil Odontol Prevent & Social, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Fis, Fac Filosofia Ciencias & Letras Ribeirao Pret, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Odontol Restauradora, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 9, p. 1153-1158, SEP 2012.
• Citações Web of Science: 17

Resumo

Objectives: Considering the enamel chemical structure, especially carbonate band, which has a major role in the caries prevention, the objective of the present study was to assess the chemical alterations on the enamel irradiated with CO2 laser by means of FTIR spectroscopy and SEM analysis. Design: The enamel surfaces were analysed on a spectrometer for acquisition of the absorption spectrum relative to the chemical composition of the control sample. The irradiation was conducted with a 10.6-mu m CO2 laser (0.55 W, 660 W/cm(2)). The carbonate absorption band at 1600-1291 cm(-1) as well as the water absorption band at 3793-2652 cm(-1) was measured in each sample after the irradiation. The water band was measured again 24-h after the irradiation. The band area of each chemical compound was delimited, the background was subtracted, and the area under each band was integrated. Each area was normalized by the phosphate band (1190-702 cm(-1)). Results: There was a statistically significant decrease (p < 0.05) in the water content after irradiation (control: 0.184 +/- 0.04; irradiated: 0.078 +/- 0.026), which increased again after rehydration (0.145 +/- 0.038). The carbonate/phosphate ratio was measured initially (0.112 +/- 0.029) and its reduction after irradiation indicated the carbonate loss (0.088 +/- 0.014) (p < 0.05). Conclusion: The 10.6-mu m CO2 laser irradiation diminishes the carbonate and water contents in the enamel after irradiation. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/06861-0 - Influência da irradiação dos lasers Nd:YAG e CO2 na composição química e ultra-estrutural do esmalte dental
• Beneficiário: Alessandra Marques Corrêa Afonso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 03/13489-6 - Caracterização de tecidos dentais duros após irradiação laser e tratamento químico: avaliação da composição, estrutura e resistência química
• Beneficiário: Luciano Bachmann
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores