Mapping of angular leaf spot resistance QTL in common bean (Phaseolus vulgaris L.) under different environments

Autor(es)

Oblessuc, Paula Rodrigues ^{[1, 2]} ; Baroni, Renata Moro ^[1] ; Franco Garcia, Antonio Augusto ^[3] ; Chioratto, Alisson Fernando ^[1] ; Morais Carbonell, Sergio Augusto ^[1] ; Aranha Camargo, Luis Eduardo ^[3] ; Benchimol, Luciana Lasry ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron IAC, BR-13012970 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao & Bioagentes, UNICAMP, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz Queiroz ESALq USP, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC GENETICS; v. 13, JUN 27 2012.
• Citações Web of Science: 18

Resumo

Background: Common bean (Phaseolus vulgaris L.) is the most important grain legume for human diet worldwide and the angular leaf spot (ALS) is one of the most devastating diseases of this crop, leading to yield losses as high as 80%. In an attempt to breed resistant cultivars, it is important to first understand the inheritance mode of resistance and to develop tools that could be used in assisted breeding. Therefore, the aim of this study was to identify quantitative trait loci (QTL) controlling resistance to ALS under natural infection conditions in the field and under inoculated conditions in the greenhouse. Results: QTL analyses were made using phenotypic data from 346 recombinant inbreed lines from the IAC-UNA x CAL 143 cross, gathered in three experiments, two of which were conducted in the field in different seasons and one in the greenhouse. Joint composite interval mapping analysis of QTL x environment interaction was performed. In all, seven QTLs were mapped on five linkage groups. Most of them, with the exception of two, were significant in all experiments. Among these, ALS10.1(DG,UC) presented major effects (R-2 between 16% - 22%). This QTL was found linked to the GATS11b marker of linkage group B10, which was consistently amplified across a set of common bean lines and was associated with the resistance. Four new QTLs were identified. Between them the ALS5.2 showed an important effect (9.4%) under inoculated conditions in the greenhouse. ALS4.2 was another major QTL, under natural infection in the field, explaining 10.8% of the variability for resistance reaction. The other QTLs showed minor effects on resistance. Conclusions: The results indicated a quantitative inheritance pattern of ALS resistance in the common bean line CAL 143. QTL x environment interactions were observed. Moreover, the major QTL identified on linkage group B10 could be important for bean breeding, as it was stable in all the environments. Thereby, the GATS11b marker is a potential tool for marker assisted selection for ALS resistance. (AU)

• Processo FAPESP: 10/51673-7 - Identificação de genes candidatos para resistência à mancha angular em feijão comum (Phaseolus vulgaris L.)
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/58332-5 - Construção de um mapa genético molecular e mapeamento de locos ligados à resistência à mancha angular em feijão comum (Phaseolus vulgaris L.) utilizando marcadores microssatélites
• Beneficiário: Paula Rodrigues Oblessuc
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/53819-0 - Mapeamento de locos ligados à resistência à antracnose e mancha angular em feijão comum utilizando marcadores microssatélites
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular