Biotransformation of Phenylacetonitrile to 2-Hydroxyphenylacetic Acid by Marine Fungi

Autor(es)

de Oliveira, Julieta Rangel ^[1] ; Mizuno, Carolina Megumi ^[2] ; Regali Seleghim, Mirna Helena ^[2] ; Diniz Javaroti, Darci Consolacao ^[2] ; Oliveira Rezende, Maria Olimpia ^[1] ; Landgraf, Maria Diva ^[1] ; Sette, Lara Duraes ^[3] ; Meleiro Porto, Andre Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Ecol & Biol Evolut, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Estadual Campinas, Div Recursos Microbianos, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, CPQBA, BR-13140000 Paulinia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE BIOTECHNOLOGY; v. 15, n. 1, p. 97-103, FEB 2013.
• Citações Web of Science: 13

Resumo

Marine fungi belonging to the genera Aspergillus, Penicillium, Cladosporium, and Bionectria catalyzed the biotransformation of phenylacetonitrile to 2-hydroxyphenylacetic acid. Eight marine fungi, selected and cultured with phenylacetonitrile in liquid mineral medium, catalyzed it quantitative biotransformation to 2-hydroxyphenylacetic acid. In this study, the nitrile group was firstly hydrolysed, and then, the aromatic ring was hydroxylated, producing 2-hydroxyphenylacetic acid with 51 % yield isolated. In addition, the 4-fluorophenylacetonitrile was exclusively biotransformed to 4-fluorophenylacetic acid by Aspergillus sydowii Ce19 (yield = 51 %). The enzymatic biotransformation of nitriles is not trivial, and here, we describe an efficient method for production of phenylacetic acids in mild conditions. (AU)

• Processo FAPESP: 08/56371-9 - Hidrolise enzimatica de nitrilas por microrganismos de origem marinha
• Beneficiário: Julieta Rangel de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado