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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Human beta-defensin 2 and protease activated receptor-2 expression in patients with chronic periodontitis

Texto completo
Autor(es):
Pereira, Alexandre Lustosa [1] ; Holzhausen, Marinella [2] ; Nobre Franco, Gilson Cesar [1] ; Cortelli, Sheila Cavalca [1] ; Cortelli, Jose Roberto [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Taubate, Dept Periodontol, Taubate, SP - Brazil
[2] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 12, p. 1609-1614, DEC 2012.
Citações Web of Science: 13
Resumo

Objective: Some previous studies have shown that gingipains, trypsin-like proteases produced by Porphyromonas gingivalis, up-regulate human beta defensin-2 (HBD-2) mRNA expression through protease-activated receptor-2 (PAR(2)) in gingival epithelial cells. This study aimed at investigating salivary HBD-2 levels and crevicular PAR(2) mRNA expression in human chronic periodontitis and evaluating whether periodontal treatment affected this process. Methods: Salivary and gingival crevicular fluid (GCF) samples were collected from periodontally healthy (control) and chronic periodontitis patients at baseline and 50 days after nonsurgical periodontal treatment. Salivary HBD-2, and GCF TNF-alpha levels were analysed by ELISA, and PAR(2) mRNA at the GCF was evaluated by RT-PCR. Results: P. gingivalis was significantly (p < 0.05) more prevalent in patients with chronic periodontitis when compared to controls. This prevalence decreased after periodontal therapy (p < 0.0001). The control group showed statistically significant lower levels of HBD-2, TNF-alpha, and PAR(2) expression when compared to the chronic periodontitis group. In addition, periodontal treatment significantly reduced PAR(2) expression and HBD-2 levels in chronic periodontitis patients (p < 0.001). Conclusions: Our results suggest that salivary HBD-2 levels and PAR(2) mRNA expression from GCF are higher in subjects with chronic periodontitis than in healthy subjects, and that periodontal treatment decreases both HBD-2 levels and PAR(2) expression. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 07/50665-8 - Associação do receptor tipo 2 ativado por protease (PAR2) com a doença periodontal em humanos
Beneficiário:Marinella Holzhausen Caldeira
Linha de fomento: Auxílio à Pesquisa - Regular