In Vivo Protective Activity of Styrax camporum Hydroalcoholic Extract against Genotoxicity Induced by Doxorubicin and Methyl Methanesulfonate in the Micronucleus and Comet Assays

Autor(es)

de Oliveira, Pollyanna Francielli ^[1] ; Furtado, Ricardo Andrade ^[1] ; Acesio, Nathalia Oliveira ^[1] ; Leandro, Luis Fernando ^[1] ; Montanheiro, Giovanna ^[1] ; de Padua, Francisnela Correa ^[1] ; Correa, Mariana Beltrame ^[1] ; Braguini, Caio Guedes ^[1] ; Pauletti, Patricia Mendonca ^[1] ; Tavares, Denise Crispim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, BR-14404600 Franca, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Planta Medica; v. 78, n. 18, p. 1899-1905, DEC 2012.
• Citações Web of Science: 2

Resumo

Styrax camporum Pohl is a tall shrub or a tree with small white flowers, which grows in the states of Sao Paulo and Minas Gerais and is popularly used for the treatment of gastroduodenal diseases. Considering this last fact, the aim of this study was to evaluate the genotoxic potential of S. camporum hydroalcoholic extract and its influence on genotoxicity induced by doxorubicin and methyl methanesulfonate in Swiss mice using the micronucleus and comet assays, respectively. The animals were treated by gavage with different doses of the extract (250, 500, and 1000 mg/kg body weight). For antigenotoxicity assessment, different doses of the S. camporum extract were administered simultaneously with doxorubicin (micronucleus test; 15 mg/kg) and methanesulfonate (comet assay; 40 mg/kg). The results showed that the S. camporum extract itself was not genotoxic in the mouse micronucleus or comet assay. The number of micronucleated polychromatic erythrocytes was significantly lower in animals treated with the S. camporum extract and doxorubicin when compared to animals treated only with doxorubicin. In the comet assay, the S. camporum extract, at the doses tested, significantly reduced the extent of DNA damage in liver cells induced by methanesulfonate. The putative activity of the active compounds of S. camporum extract may explain the effect of this plant on genotoxicity induced by doxorubicin and methanesulfonate. (AU)

• Processo FAPESP: 11/21310-2 - ESTUDO DAS ATIVIDADES CITOTÓXICA, GENOTÓXICA E ANTIOXIDANTE DO EXTRATO HIDROALCOÓLICO DE Styrax camporum E DOS SEUS MARCADORES QUÍMICOS, EGONOL E HOMOEGONOL, E DA SUA INFLUÊNCIA SOBRE OS DANOS NO MATERIAL GENÉTICO E LESÕES PRÉ-NEOPLÁSICAS
• Beneficiário: Pollyanna Francielli de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/10283-1 - Estudo fitoquímico e avaliação da mutagenicidade, antimutagenicidade e da atividade inibitória da ciclooxigenase de Styrax (Styracaceae)
• Beneficiário: Patricia Mendonça Pauletti
• Modalidade de apoio: Auxílio à Pesquisa - Regular