Phylogenetic and structural studies of a novel equine papillomavirus identified from aural plaques

Autor(es)

Taniwaki, Sueli A. ^[1] ; Magro, Angelo J. ^[2] ; Gorino, Ana Claudia ^[3] ; Oliveira-Filho, Jose P. ^[3] ; Fontes, Marcos R. M. ^[2] ; Borges, Alexandre S. ^[3] ; Araujo, Jr., Joao P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Biosci Inst, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Biosci Inst, Dept Phys & Biophys, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Coll Vet Med & Anim Sci, Dept Vet Clin Sci, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 162, n. 1, p. 85-93, FEB 22 2013.
• Citações Web of Science: 9

Resumo

Papillomaviruses (PVs) infect a wide range of animal species and show great genetic diversity. To date, excluding equine sarcoids, only three species of PVs were identified associated with lesions in horses: Equus caballus papillomavirus 1 (EcPV1-cutaneous), EcPV2 (genital) and EcPV3 (aural plaques). In this study, we identified a novel equine PV from aural plaques, which we designated EcPV4. Cutaneous samples from horses with lesions that were microscopically diagnosed as aural plaques were subjected to DNA extraction, amplification and sequencing. Rolling circle amplification and inverse PCR with specific primers confirmed the presence of an approximately 8 kb circular genome. The full-length EcPV4 L1 major capsid protein sequence has 1488 nucleotides (495 amino acids). EcPV4 had a sequence identity of only 53.3%, 60.2% and 51.7% when compared with the published sequences for EcPV1, EcPV2 and EcPV3, respectively. A Bayesian phylogenetic analysis indicated that EcPV4 clusters with EcPV2, but not with EcPV1 and EcPV3. Using the current PV classification system that is based on the nucleotide sequence of L1, we could not define the genus of the newly identified virus. Therefore, a structural analysis of the L1 protein was carried out to aid in this classification because EcPV4 cause lesion similar to the lesion caused by EcPV3. A comparison of the superficial loops demonstrated a distinct amino acid conservation pattern between EcPV4/EcPV2 and EcPV4/EcPV3. These results demonstrate the presence of a new equine PV species and that structural studies could be useful in the classification of PVs. (C) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/52718-7 - Clonagem e expressão de diferentes proteínas do vírus da imunodeficiência felina para utilização no ELISA indireto e imunocromatografia para detecção de anticorpos
• Beneficiário: João Pessoa Araújo Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular