Cell viability and adhesion on diamond-like carbon films containing titanium dioxide nanoparticles

Autor(es)

Wachesk, C. C. ^[1] ; Pires, C. A. F. ^[1] ; Ramos, B. C. ^[2] ; Trava-Airoldi, V. J. ^[3] ; Lobo, A. O. ^[2] ; Pacheco-Soares, C. ^[1] ; Marciano, F. R. ^[2] ; Da-Silva, N. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba Univap, Lab Biomed Nanotechnol NanoBio, Lab Biol Celular & Tecidual, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba Univap, Lab Biomed Nanotechnol NanoBio, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[3] INPE, Associated Lab Sensors & Mat, BR-12227010 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Surface Science; v. 266, p. 176-181, FEB 1 2013.
• Citações Web of Science: 19

Resumo

The combination of low friction, wear resistance, high hardness, biocompatibility and chemical inertness makes diamond-like carbon (DLC) films suitable in a numerous applications in biomedical engineering. The cell viability and adhesion of L929 mouse fibroblasts was investigated using two different colorimetric assays: (i) 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (MTT), and (ii) lactate dehydrogenase (LDH). The films were growth on 316L stainless steel substrates using plasma enhanced chemical vapor deposition technique from a dispersion of TiO2 nanopowder in hexane. The increasing concentration of TiO2 nanoparticles in DLC films enhanced the mitochondrial activity and decreases the LDH activity on these samples. Fluorescence and scanning electron microscopy corroborate the results. These experiments show the potential use of DLC and TiO2-DLC films in biomedical applications. (c) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores