Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti

Texto completo
Autor(es):
Costa-da-Silva, Andre Luis [1] ; Navarrete, Flavia Rosa [1] ; Salvador, Felipe Scassi [2] ; Karina-Costa, Maria [1] ; Ioshino, Rafaella Sayuri [1] ; Azevedo, Diego Soares [1] ; Rocha, Desiree Rafaela [1] ; Romano, Camila Malta [2] ; Capurro, Margareth Lara [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo - Brazil
[2] Univ Sao Paulo, Virol Lab, Inst Trop Med, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 8, n. 1 JAN 14 2013.
Citações Web of Science: 14
Resumo

Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated ``Glytube{''} was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti. (AU)

Processo FAPESP: 08/10254-1 - Promovendo mortalidade em Aedes aegypti infectado pelo vírus dengue
Beneficiário:Margareth de Lara Capurro-Guimarães
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/53637-0 - Interação Anopheles-Plasmodium: uma abordagem multidisciplinar
Beneficiário:Margareth de Lara Capurro-Guimarães
Linha de fomento: Auxílio à Pesquisa - Temático