Antimicrobial Activity of Triantibiotic Paste, 2% Chlorhexidine Gel, and Calcium Hydroxide on an Intraoral-infected Dentin Biofilm Model

Autor(es)

Ordinola-Zapata, Ronald ^[1] ; Bramante, Clovis M. ^[1] ; Minotti, Paloma Gagliardi ^[1] ; Cavenago, Bruno Cavalini ^[1] ; Garcia, Roberto Brandao ^[1] ; Bernardineli, Norberti ^[1] ; Jaramillo, David E. ^[2] ; Duarte, Marco A. Hungaro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Dent Sch Bauru, Dept Endodont, BR-05508 Sao Paulo - Brazil

^[2] Loma Linda Sch Dent, Dept Endodont, Loma Linda, CA - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 39, n. 1, p. 115-118, JAN 2013.
• Citações Web of Science: 28

Resumo

Introduction: The purpose of this study was to evaluate the antimicrobial activity of calcium hydroxide, 2% chlorhexidine gel, and triantibiotic paste (ie, metronidazole, minocycline, and ciprofloxacin) by using an intraorally infected dentin biofilm model. Methods: Forty bovine dentin specimens were infected intraorally using a removable orthodontic device in. order to induce the biofilm colonization of the dentin. Then, the samples were treated with the medications for 7 days. Saline solution was used as the control. Two evaluations were performed: immediately after the elimination of the medication and after incubation in brain-heart infusion medium for 24 hours. The Live/Dead technique (Invitrogen, Eugene, OR) and a confocal microscope were used to obtain the percentage of live cells. Nonparametric statistical tests were performed to show differences in the percentage of live cells among the groups (P < .05). Results: Calcium hydroxide and 2% chlorhexidine gel did not show statistical differences in the immediate evaluation. However, after application of the brain-heart infusion medium for 24 hours, 2% gel chlorhexidine showed a statistically lesser percentage of live cells in comparison with calcium hydroxide. The triantibiotic paste significantly showed a lower percentage of live cells in comparison with the 2% chlorhexidine gel and calcium hydroxide groups in the immediate and secondary (after 24 hours) evaluations. Conclusions: The triantibiotic paste was most effective at killing the bacteria in the biofilms on the intraorally infected dentin model in comparison with 2% chlorhexidine gel and calcium hydroxide. (J Endod 2013;39:115-118) (AU)

• Processo FAPESP: 10/16002-4 - Efeito das soluções químicas e dos meios físicos utilizados na irrigação endodôntica na dentina infectada por biofilmes orais
• Beneficiário: Ronald Ordinola Zapata
• Modalidade de apoio: Bolsas no Brasil - Doutorado