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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial Activity of Triantibiotic Paste, 2% Chlorhexidine Gel, and Calcium Hydroxide on an Intraoral-infected Dentin Biofilm Model

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Author(s):
Ordinola-Zapata, Ronald [1] ; Bramante, Clovis M. [1] ; Minotti, Paloma Gagliardi [1] ; Cavenago, Bruno Cavalini [1] ; Garcia, Roberto Brandao [1] ; Bernardineli, Norberti [1] ; Jaramillo, David E. [2] ; Duarte, Marco A. Hungaro [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Bauru Dent Sch Bauru, Dept Endodont, BR-05508 Sao Paulo - Brazil
[2] Loma Linda Sch Dent, Dept Endodont, Loma Linda, CA - USA
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 39, n. 1, p. 115-118, JAN 2013.
Web of Science Citations: 28
Abstract

Introduction: The purpose of this study was to evaluate the antimicrobial activity of calcium hydroxide, 2% chlorhexidine gel, and triantibiotic paste (ie, metronidazole, minocycline, and ciprofloxacin) by using an intraorally infected dentin biofilm model. Methods: Forty bovine dentin specimens were infected intraorally using a removable orthodontic device in. order to induce the biofilm colonization of the dentin. Then, the samples were treated with the medications for 7 days. Saline solution was used as the control. Two evaluations were performed: immediately after the elimination of the medication and after incubation in brain-heart infusion medium for 24 hours. The Live/Dead technique (Invitrogen, Eugene, OR) and a confocal microscope were used to obtain the percentage of live cells. Nonparametric statistical tests were performed to show differences in the percentage of live cells among the groups (P < .05). Results: Calcium hydroxide and 2% chlorhexidine gel did not show statistical differences in the immediate evaluation. However, after application of the brain-heart infusion medium for 24 hours, 2% gel chlorhexidine showed a statistically lesser percentage of live cells in comparison with calcium hydroxide. The triantibiotic paste significantly showed a lower percentage of live cells in comparison with the 2% chlorhexidine gel and calcium hydroxide groups in the immediate and secondary (after 24 hours) evaluations. Conclusions: The triantibiotic paste was most effective at killing the bacteria in the biofilms on the intraorally infected dentin model in comparison with 2% chlorhexidine gel and calcium hydroxide. (J Endod 2013;39:115-118) (AU)

FAPESP's process: 10/16002-4 - On the chemichal and physical endodontic methods to decontaminate intraorally infected dentin
Grantee:Ronald Ordinola Zapata
Support Opportunities: Scholarships in Brazil - Doctorate