Evaluation of the effect of intracanal medications used in pulp revascularization/revitalization on the push-out bond strength of MTA Angelus white® and Biodentine®

• Grant number: 16/10889-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2016
• Effective date (End): July 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Adriana de Jesus Soares
• Grantee: Mariana Valerio Pallone
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The endodontic treatment of teeth with incomplete root formation is a challenge due to the presence of open apices and thin and fragile dentinal walls which may lead to fractures. Treatment options involve apexification with intracanal medication changes and MTA plug in the apical region and has recently emerged a new alternative that is the pulp revitalization/ revascularization procedure. The aim of this study is to evaluate the effect of three intracanal medications on the push-out bond strength of MTA Angelus white® and Biodentine ®, these latter being used as cervical sealings in revascularization procedure. Extracted human mandibular single-rooted premolars (n=70) will be selected premolars and divided into 7 groups of 10 teeth each, according to the cervical sealing and intracanal medication, and this will remain for 21 days. The groups are: Group 1: treated with calcium hydroxide teeth powder and 2% chlorhexidine gel and sealed with MTA Angelus white®; Group 2: treated with calcium hydroxide teeth powder and 2% chlorhexidine gel and sealed with Biodentine®; Group 3: teeth treated with calcium hydroxide powder and distilled water and sealed with MTA Angelus white®; Group 4: teeth treated with calcium hydroxide powder and distilled water and sealed with Biodentine®; Group 5: teeth treated with triple antibiotic paste (metronidazole, ciprofloxacin and minocycline) and sealed with MTA Angelus white®; Group 6: teeth treated with triple antibiotic paste and sealed with Biodentine®; Group 7: control group (no intracanal medication), 5 teeth sealed with MTA Angelus white® and 5 teeth sealed with Biodentine®. Subsequently, the specimens are subjected to thermomechanical cycling test to simulate one year of clinical condition and thereupon be transversely severed in the cervical third to be analyzed by the push-out test in a universal testing machine. The results will be analyzed statistically at a significance level of 5%.