The purpose of this study is to evaluate the push-out bond strength of bioactive materials used for cervical sealing after different medications in revitalized teeth. Ten permanent maxillary central incisors will be selected and the cervical and apical third will be removed. The middle third will be horizontally cut, obtaining four 1 mm thick (± 0.1 mm) slices of each incisor. Following this, 3 holes with 0.8 diameters will be drilled in each slice. Samples will be randomly distributed in 12 groups (n=10), according to the intracanal medication and cervical sealing used. Specimens will receive standardized irrigation and then they will be dried with absorbent paper. The 4 consecutive slices of one single root will be filled with one of the tested medications: calcium hydroxide associated with distilled water; calcium hydroxide associated with 2% chlorhexidine gel; double antibiotic paste (ciprofloxacin and metronidazole); or triple antibiotic paste (ciprofloxacin, metronidazole and minocycline). After 21 days in a greenhouse at 37°C, the medications will be removed and, in the same slice, each drilled hole will be filled with one of the sealer materials tested: White MTA, MTA HP or Endosequence Bioceramic Root Repair Material. After that, filled slices will be stored in a greenhouse under the temperature of 37ºC for 7 days before accomplishing push-out assay. Load will be applied at a speed of 0,5 mm/min until material displacement, being the results expressed in MPa. The mean and standard deviation of values will be calculated for each group and data will be statistically analyzed. The statistical tests to be used will be determined after the evaluation of the obtained data.
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