Photodynamic Inactivation of Planktonic Cultures and Biofilms of Candida albicans Mediated by Aluminum-Chloride-Phthalocyanine Entrapped in Nanoemulsions

Autor(es)

Dias Ribeiro, Ana Paula ^[1] ; Andrade, Mariana Carvalho ^[1] ; da Silva, Julhiany de Fatima ^[2] ; Jorge, Janaina Habib ^[1] ; Primo, Fernando Lucas ^[3] ; Tedesco, Antonio Claudio ^[3] ; Pavarina, Ana Claudia ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Dept Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Jaboticabal, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP - Brazil

^[3] FFCLRP Sao Paulo Univ, Ctr Nanotechnol & Tissue Engineers, Photobiol & Photomed Res Grp, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 89, n. 1, p. 111-119, JAN-FEB 2013.
• Citações Web of Science: 22

Resumo

New drug delivery systems, such as nanoemulsions (NE), have been developed to allow the use of hydrophobic drugs on the antimicrobial photodynamic therapy. This study evaluated the photodynamic potential of aluminum-chloride-phthalocyanine (ClAlPc) entrapped in cationic and anionic NE to inactivate Candida albicans planktonic cultures and biofilm compared with free ClAlPc. Fungal suspensions were treated with different delivery systems containing ClAlPc and light emitting diode. For planktonic suspensions, colonies were counted and cell metabolism was evaluated by XTT assay. Flow cytometry evaluated cell membrane damage. For biofilms, the metabolic activity was evaluated by XTT and ClAlPc distribution through biofilms was analyzed by confocal laser scanning microscopy (CLSM). Fungal viability was dependent on the delivery system, superficial charge and light dose. Free ClAlPc caused photokilling of the yeast when combined with 100 J cm-2. Cationic NE-ClAlPc reduced significantly both colony counts and cell metabolism (P < 0.05). In addition, cationic NE-ClAlPc and free ClAlPc caused significant damage to the cell membrane (P < 0.05). For the biofilms, cationic NE-ClAlPc reduced cell metabolism by 70%. Anionic NE-ClAlPc did not present antifungal activity. CLSM showed different accumulation on biofilms between the delivery systems. Although NE system showed a lower activity for planktonic culture, cationic NE-ClAlPc showed better results for Candida biofilms. (AU)

• Processo FAPESP: 08/03994-9 - Estudo da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado nas células fúngicas: avaliação in vitro e in vivo de dois fotossensibilizadores
• Beneficiário: Ana Cláudia Pavarina
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05425-1 - Avaliação da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado a células epiteliais: avaliação in vitro e in vivo da cloro alumínio ftalocianina e e outros derivados
• Beneficiário: Ana Cláudia Pavarina
• Modalidade de apoio: Auxílio à Pesquisa - Regular