Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs

Autor(es)

Carolina V. Uliana ^[1] ; Carla S. Riccardi ^[2] ; João O. Tognolli ^[3] ; Hideko Yamanaka ^[4]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista. Instituto de Química - Brasil

^[2] Universidade Estadual Paulista. Instituto de Química - Brasil

^[3] Universidade Estadual Paulista. Instituto de Química - Brasil

^[4] Universidade Estadual Paulista. Instituto de Química - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 19, n. 4, p. 782-787, 2008-00-00.

Resumo

Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system. (AU)

• Processo FAPESP: 04/09535-5 - Desenvolvimento de genossensor para deteccao do virus da hepatite c.
• Beneficiário: Carolina Venturini Uliana
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica