Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs

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Author(s):	Carolina V. Uliana ^[1] ; Carla S. Riccardi ^[2] ; João O. Tognolli ^[3] ; Hideko Yamanaka ^[4] Total Authors: 4
Affiliation:	^[1] Universidade Estadual Paulista. Instituto de Química - Brasil ^[2] Universidade Estadual Paulista. Instituto de Química - Brasil ^[3] Universidade Estadual Paulista. Instituto de Química - Brasil ^[4] Universidade Estadual Paulista. Instituto de Química - Brasil Total Affiliations: 4
Document type:	Journal article
Source:	Journal of the Brazilian Chemical Society; v. 19, n. 4, p. 782-787, 2008-00-00.
Abstract
Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system. (AU)