Transmission of Aggregatibacter actinomycetemcomitans between Brazilian women with severe chronic periodontitis and their children

Autor(es)

Rodrigo Otávio Citó César Rêgo ^[1] ; Denise Madalena Palomari Spolidorio ^[2] ; Sérgio Luiz de Souza Salvador ^[3] ; Joni Augusto Cirelli ^[4]

Afiliação do(s) autor(es)

^[1] Federal University of Ceará. School of Pharmacy, Dentistry and Nursing. Department of Clinical Dentistry - Brasil

^[2] São Paulo State University. School of Dentistry of Araraquara. Department of Physiology and Pathology - Brasil

^[3] São Paulo State University. School of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical, Toxicologic and Bromatologic Analysis - Brasil

^[4] São Paulo State University. School of Dentistry of Araraquara. Department of Diagnosis and Surgery - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 18, n. 3, p. 220-224, 2007-00-00.

Resumo

This study evaluated the transmission of Aggregatibacter actinomycetemcomitans (Aa) in women with severe chronic periodontitis and their children. Thirty women (mean age = 36.1±6.0 years) who were mothers of at least one child aged 7 to 16 years were enrolled. In order to investigate mother-child transmission of Aa, the children were also evaluated when their mothers were colonized by the bacterium. Subgingival plaque samples of each woman were collected from 3 sites (mean probing depth of 7.3±1.2 mm and mean clinical attachment level of 7.9±1.5 mm) and pooled in reduced transport fluid (RTF). These samples were processed, inoculated onto TSBV-agar selective medium and incubated at 37°C in microaerophilic atmosphere for 5 days. Aa was identified on the basis of colony morphology, Gram staining, catalase and oxidase reactions. Aa was found in 8 out of 30 women. Therefore, 8 children from these women (mean age= 12 ± 3.7 years) were evaluated, but Aa was found only in 2 of them. Aa strains of the two mother-child pairs were evaluated by arbitrarily-primed polymerase chain reaction (AP-PCR), although it was not found similarity between the amplitypes of each pair. No Aa transmission was found between Brazilian women with severe chronic periodontitis and their children. (AU)

• Processo FAPESP: 00/08658-5 - Avaliação das condições clínicas e microbiológicas periodontais e da transmissão de Actinobacillus actinomycetemcomitans entre mães e filhos
• Beneficiário: Joni Augusto Cirelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular