Apoptosis in Denervated Skeletal Muscle

Autor(es)

Giovanna Castilho Davatz ^[1] ; Jesus Carlos Andreo ^[2] ; Antonio de Castro Rodrigues ^[3] ; Geraldo Marco Rosa Júnior ; Luiz Henrique Rapucci Moraes

Afiliação do(s) autor(es)

^[1] USP. FOB. Department of Fonoaudiology - Brasil

^[2] USP. FOB. Department of Biological Sciences - Brasil

^[3] USP. FOB. Department of Biological Sciences - Brasil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MORPHOLOGY; v. 25, n. 3, p. 529-536, 2007-09-00.

Resumo

The apoptosis phenomenon happens in normal tissues and in many pathological conditions as described by several researchers. Most studied in the 70s, this phenomenon remained almost forgotten in the 90s, when, with the advent of more advanced techniques, returned to laboratories. Thus, electronic microscopy, Comet Assays and histochemistry techniques, among others, were utilized in an effort to elucidate in a thorough way, the mechanisms that lead the cell to "suicide". No one can deny the importance of the knowledge of such phenomenon, so as to control its induction or blocking in the treatment of pathologies such as cancer. In this work, the apoptosis study was aimed at denervated muscles in different periods. Furthermore, two types of skeletal striated muscles were compared: the one called red muscle (soleo) which is resistant to fatigue, having a slow contraction, and the one named white muscle (EDL) of precocious fatigue and fast contraction. Previoulsy carried out in the techniques mentioned above, this work has been accomplished using the TUNEL technique, which is most accepted in the identification of apoptosis. It was demonstrated that this phenomenon takes place in a more marked way, in the beginning of the denervation process. In addition, it was observed that the red muscle responds more intensely to denervation than the white muscle. One can infer that the removal of the main cell function, the contraction, in the case of the skeletal striated muscle, triggers the chain reactions that culminate in apoptosis. Other projects are in process using mutant animals aiming at studying degenerative muscular illnesses and correlating them with denervation (AU)

• Processo FAPESP: 05/02609-6 - Apoptose em músculo estriado esquelético desnervado
• Beneficiário: Giovanna Castilho Davatz Lopes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica