On-line LC/UV/MS analysis of flavonols in the three apple varieties most widely cultivated in Brazil

Autor(es)

Luciana A. Tiberti ^[1] ; Janete H. Yariwake ^[2] ; Karine Ndjoko ^[3] ; Kurt Hostettmann ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil

^[2] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil

^[3] Université de Genève. École de Pharmacie Genève-Lausanne. Laboratoire de Pharmacognosie et Phytochimie - Suíça

^[4] Université de Genève. École de Pharmacie Genève-Lausanne. Laboratoire de Pharmacognosie et Phytochimie - Suíça

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 18, n. 1, p. 100-105, 2007-00-00.

Resumo

This work describes the first detailed investigation into the chemical structures of flavonols present in the three apple varieties most commonly cultivated in Brazil: Gala, Golden and Fuji. The analyses were carried out by LC/UV/MS (high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry), using post-column addition of UV shift reagents, as well as multiple stage mass spectrometry (MSn) with electrospray ionization in the negative ion mode. Rutin, hyperoside, isoquercitrin, quercitrin, quercetin and three other quercetin-3-pentoside derivatives were identified through the characterization of on-line-based LC/UV/MS data. LC/UV/MS analysis also revealed that the three apple cultivars have similar chromatographic profiles. (AU)

• Processo FAPESP: 98/10271-0 - Identificacao de flavonoides em plantas medicinais e alimenticias empregando metodos cromatograficos de analise (lc-ms, hplc-uv/dad e outros).
• Beneficiário: Luciana Aparecida Tiberti Sercheli
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 02/00493-2 - Estudo cromatografico e da atividade antioxidante de flavonoides em plantas medicinais e alimenticias brasileiras: padronizacao de medicamentos fitoterapicos e avaliacao de novos antioxidantes naturais
• Beneficiário: Janete Harumi Yariwake
• Modalidade de apoio: Auxílio à Pesquisa - Regular