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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

On-line LC/UV/MS analysis of flavonols in the three apple varieties most widely cultivated in Brazil

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Author(s):
Luciana A. Tiberti [1] ; Janete H. Yariwake [2] ; Karine Ndjoko [3] ; Kurt Hostettmann [4]
Total Authors: 4
Affiliation:
[1] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil
[2] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil
[3] Université de Genève. École de Pharmacie Genève-Lausanne. Laboratoire de Pharmacognosie et Phytochimie - Suíça
[4] Université de Genève. École de Pharmacie Genève-Lausanne. Laboratoire de Pharmacognosie et Phytochimie - Suíça
Total Affiliations: 4
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 18, n. 1, p. 100-105, 2007-00-00.
Abstract

This work describes the first detailed investigation into the chemical structures of flavonols present in the three apple varieties most commonly cultivated in Brazil: Gala, Golden and Fuji. The analyses were carried out by LC/UV/MS (high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry), using post-column addition of UV shift reagents, as well as multiple stage mass spectrometry (MSn) with electrospray ionization in the negative ion mode. Rutin, hyperoside, isoquercitrin, quercitrin, quercetin and three other quercetin-3-pentoside derivatives were identified through the characterization of on-line-based LC/UV/MS data. LC/UV/MS analysis also revealed that the three apple cultivars have similar chromatographic profiles. (AU)