Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture

Autor(es)

Fabiana R. X. Batista ^[1] ; Carlos A. Pereira ^[2] ; Ronaldo Z. Mendonça ^[3] ; Angela M. Moraes ^[4]

Afiliação do(s) autor(es)

^[1] State University of Campinas. School of Chemical Engineering. Department of Biotechnological Processes - Brasil

^[2] Butantan Institute. Laboratory of Viral de Immunology - Brasil

^[3] Butantan Institute. Laboratory of Viral de Immunology - Brasil

^[4] State University of Campinas. School of Chemical Engineering. Department of Biotechnological Processes - Brasil

• Tipo de documento: Artigo Científico
• Fonte: ELECTRONIC JOURNAL OF BIOTECHNOLOGY; v. 9, n. 5, p. 0-0, 2006-10-15.

Resumo

Insect cell culture has become increasingly useful for the production of heterologous proteins as well as of baculovirus polyhedra, and several different culture media formulations can be employed for this purpose. The goal of this work was to assess the potential of lyophylized milk whey ultra filtration retentate (MWR) when associated to yeast extract (YE), glucose and Pluronic F68 (PF68) to partially replace fetal calf serum (FBS) in Spodoptera frugiperda Sf9 cells culture in Grace's medium, aiming AgMNPV baculovirus production. Batch cultivation results showed that the yeast extract and the milk whey concentrate effectively increased cell concentration to about half the level commonly verified for the Sf900II serum-free medium. A 4-fold increase in viable cell concentration was achieved when employing 5% (w/v) MWR, 8 g/L YE, 1% (v/v) of FBS and 2.7 g.l-1 of glucose, resulting in 1.6 x 10(7) polyhedra.ml-1 after infection with baculovirus. (AU)

• Processo FAPESP: 02/09482-3 - Expressão de genes heterólogos em células de dípteros: biologia molecular e engenharia de processos
• Beneficiário: Carlos Augusto Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático