Macrophage activity and histopathology of the lymphohematopoietic organs in male Wistar rats orally exposed to single or mixed pesticides

Autor(es)

De Camargo, Marcela Rodrigues ^[1] ; Barbisan, Luis Fernando ^[1] ; Martinez, Meire Franca ^[2] ; Da Silva Franchi, Carla Adriene ^[2] ; Viana De Camargo, Joao Lauro ^[2] ; Tozzi Spinardi-Barbisan, Ana Lucia ^[2]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Inst Biosci, Dept Morphol, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN; v. 48, n. 7, p. 607-613, JUL 1 2013.
• Citações Web of Science: 3

Resumo

The noxious effects of low or effective dose exposure to single or mixed pesticides on macrophage activity and the lymphohematopoietic organs were investigated. Male Wistar rats were orally exposed to dichlorvos, dicofol, endosulfan, dieldrin and permethrin, either as single or combined mixtures during a 28-day study containing eight groups: one group received a semipurified diet (non-treated); two groups received a semipurified diet containing low dose mixture (dieldrin 0.025mg/kg, endosulfan, 0.6mg/kg, dicofol 0.22mg/kg, dichlorvos 0.23mg/kg, permethrin 5mg/kg) or an effective dose mixture (dichlorvos 2.3mg/kg, dicofol 2.5mg/kg, endosulfan 2.9mg/kg, dieldrin 0.05mg/kg and permethrin 25.0mg/kg), respectively; the other five groups received a semipurified diet containing each single pesticide in effective doses. At sacrifice, the thymus, spleen, mesenteric lymph nodes, Payer's patches and bone marrow were removed for histological analysis. Peritoneal macrophages were obtained to determine the phagocytosis and spreading indexes and tumoral necrosis factor alpha (TNF-), nitric oxide (NO) and H2O2 production. Exposure to pesticide mixtures did not alter the percentage of macrophage phagocytosis and spreading, TNF- production or the NO and H2O2 release when compared to the non-treated group. Neither was there any apparent evidence that a pesticide mixture at low or effective doses altered the histological structure of the lymphohematopoietic organs. The findings indicate that short-term treatment with pesticide mixtures did not induce an apparent immunotoxic effect in male Wistar rats. (AU)

• Processo FAPESP: 06/60506-1 - Praguicidas agrícolas como fator de risco: avaliações toxicopatológica, imunológica, molecular e analítica em modelos experimentais de exposição única e combinada
• Beneficiário: João Lauro Viana de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Temático