Digestive enzyme activity in juvenile Nile tilapia (Oreochromis niloticus, L) submitted to different dietary levels of shrimp protein hydrolysate

Autor(es)

Santos, Juliana Ferreira ^{[1, 2, 3]} ; Castro, Patricia Fernandes ^[4] ; Goncalves Leal, Albino Luciani ^[5] ; Vasconcelos de Freitas Junior, Augusto Cezar ^{[1, 2]} ; Lemos, Daniel ^[6] ; Carvalho, Jr., Luiz Bezerra ^{[1, 2]} ; Bezerra, Ranilson Souza ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Pernambuco, Dept Bioquim, Lab Enzimol LABENZ, BR-50670420 Recife, PE - Brazil

^[2] Univ Fed Pernambuco, LIKA, BR-50670420 Recife, PE - Brazil

^[3] Univ Fed Rural Pernambuco, Unidade Acad Serra Talhada, BR-56900000 Serra Talhada, PE - Brazil

^[4] Embrapa Meio Norte, BR-64200970 Parnaiba, PI - Brazil

^[5] CHESF, BR-50761901 Recife, PE - Brazil

^[6] Univ Sao Paulo, Inst Oceanog, LAM, BR-05508120 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AQUACULTURE INTERNATIONAL; v. 21, n. 3, p. 563-577, JUN 2013.
• Citações Web of Science: 11

Resumo

The effect of different dietary concentrations of shrimp protein hydrolysate (SPH) on digestive enzyme activity of Nile tilapia juveniles was evaluated. SPH concentrations in diets were 0, 15, 30 and 60 g kg(-1) (treatments SPH0, SPH15, SPH30 and SPH60, respectively). Hemoglobin, azocasein, BApNA (N alpha-benzoyl-dl-arginine-p-nitroanilide), SApNA (Suc-Ala-Ala-Pro-Phe p-nitroanilide), aminoacyl of beta-naphthylamide and starch were used as substrates for enzyme activity determinations. The activity of total alkaline protease was significantly higher (P < 0.05) in fish under SPH15 and SPH60 treatments than in the control (SPH0). However, the effect was not dose-dependent. Substrate-SDS-PAGE was also performed to evaluate changes in the profile of Nile tilapia digestive proteases caused by SPH. Substrate-SDS-PAGE revealed 12 active proteolytic bands, eight of which responded to SPH dietary incorporation. Inhibition substrate-SDS-PAGE indicated a decrease in the activity of three enzymes, with trypsin activity decreasing with the increase of SPH concentration, whereas the opposite occurred for two aminopeptidases. Distinct protease profiles were also found for each treatment, suggesting adaptability of digestive proteases from Nile tilapia to the different diets. (AU)

• Processo FAPESP: 07/07051-9 - Digestibilidade in vitro de proteína utilizando enzimas espécie específicas: potencial de identificação de ingredientes de reação sustentáveis para o cultivo de camarões marinhos (Sustain-Ing)
• Beneficiário: Daniel Eduardo Lavanholi de Lemos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/50578-2 - Testando a qualidade de racoes para o cultivo de camaroes marinhos: validacao do metodo in vitro ph-stat por meio de ensaios in vivo de digestibilidade proteica (ezfeed).
• Beneficiário: Daniel Eduardo Lavanholi de Lemos
• Modalidade de apoio: Auxílio à Pesquisa - Regular