Leucine-Rich Diet Improves the Serum Amino Acid Profile and Body Composition of Fetuses from Tumor-Bearing Pregnant Mice

Autor(es)

Viana, Lais Rosa ^[1] ; Gomes-Marcondes, Maria Cristina C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Lab Nutr & Canc, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGY OF REPRODUCTION; v. 88, n. 5 MAY 2013.
• Citações Web of Science: 11

Resumo

Pregnancy is a complex process that can be jeopardized when associated with cancer, because of the coexistence of two complex metabolic conditions: a fetus and cancer. The aim of this study was to evaluate fetal growth in association with cancer development as well as the indirect effects produced by tumors in pregnant mice subjected to a leucine-rich diet, knowing that leucine supplementation can minimize the tumor effects by acting as a cell signaling agent to improve the protein synthesis process. We evaluated fetuses (n = 6) from NMRI pregnant mice fed either a control or a leucine-rich diet in either the presence or absence of an MAC16 colon adenocarcinoma or ascitic fluid inoculation. The fetal serum amino acids were separated using high-performance liquid chromatography, and fetal cytokine levels were analyzed using a microsphere-based multiplex immunoassay (Luminex xMAP). Fetal body composition was measured as the water, fat, and protein total content and total serum protein, albumin, and glucose content. Tumor growth resulted in a severe reduction in fetal body weight and protein content and increased fetal resorption, associated with placental weight decrease; these effects were minimized by a leucine-rich diet. Serum total protein and glucose content were reduced in fetuses from tumor-bearing dams but were reverted by nutritional supplementation. The serum amino acid profiles differed significantly between the tumor-bearing mice fed with a leucine-rich diet and controls. Certain tumor effects were reproduced in fetuses from ascitic fluid-injected dams, suggesting indirect effects of tumor growth. We conclude that certain effects of tumor growth can be mimicked by ascitic fluid injection and can be modulated by a leucine-rich diet. (AU)

• Processo FAPESP: 12/06955-0 - Avaliação do perfil metabolômico, proteômico, das vias de proliferação celular e apoptose do câncer sob influência dos hormônios da gravidez em ratas submetidas à dieta rica em leucina
• Beneficiário: Laís Rosa Viana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/00209-9 - Nutrição e câncer estudo das alterações metabólicas e moleculares do metabolismo protéico no câncer-caquexia, moduladas pela suplementação nutricional com leucina, no crescimento, desenvolvimento e gravidez
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/11982-3 - Avaliação do perfil de aminoácidos fetal e materno e atividade placentária em camundongas NMRI portadoras do adenocarcinoma de cólon (MAC16)
• Beneficiário: Laís Rosa Viana
• Modalidade de apoio: Bolsas no Brasil - Mestrado