| Texto completo | |
| Autor(es): |
Priolli, Denise G.
[1]
;
Canelloi, Thamy P.
[2]
;
Lopes, Camila O.
[2]
;
Valdivia, Julio C. M.
[2]
;
Martinez, Natalia P.
[2]
;
Acari, Demetrius P.
[2]
;
Cardinalli, Izilda A.
[3]
;
Ribeiro, Marcelo L.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Francisco, Sch Med, Postgrad Program Strictu Senso Hlth Sci, Sao Paulo - Brazil
[2] Univ Sao Francisco, Sch Med, Sao Paulo - Brazil
[3] Univ Sao Francisco, Sch Med, Dept Pathol, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF COLORECTAL DISEASE; v. 28, n. 5, p. 713-722, MAY 2013. |
| Citações Web of Science: | 4 |
| Resumo | |
Oxidative DNA damage is one of the mechanisms associated to initial colorectal carcinogenesis, but how it interacts with beta-catenin, an adherence protein related to cancer evolution, is not clear. This study investigates the relationship between oxidative DNA damage and beta-catenin expression in normal mucosa and colon tumor tissue (adenoma and adenocarcinoma) in colorectal adenocarcinoma evolution. One hundred and 13 samples were studied. Hematoxylin-eosin determined histological grade. beta-Catenin expression was analyzed by immunohistochemistry. The oxidative DNA damage was evaluated using comet assay technique. The coefficient for rejection of the nullity hypothesis was taken to 5 %. Kruskal-Wallis, Spearman test, and partial correlation were used to analyze the data. There was oxidative DNA damage increase in colorectal cancer evolution (p < 0.01). Histological grade was correlated with oxidative DNA damage (p < 0.01). There were differences in beta-catenin expression among normal, adenoma, and adenocarcinoma tissue with progressive increase of beta-catenin expression (p < 0.00). Histological grade was correlated to beta-catenin expression (p < 0.00). There was a relationship (p < 0.00) between beta-catenin and histological grade while controlling for the effect of oxidative DNA damage. The findings of this study make it possible to establish a relationship between oxidative DNA damage and beta-catenin expression in normal mucosa and colorectal tumor tissue. Additionally, they show a causal relationship between variations of beta-catenin in different tissues analyzed while controlling for the effect of oxidative DNA damage. (AU) | |
| Processo FAPESP: | 07/01196-5 - Relação entre estresse oxidativo e classes morfofuncionais do adenocarcinoma colorretal avaliada pela expressão tecidual das proteínas de adesão celular (CEA, E-caderina, beta-catenina) e pela dinâmica do antígeno carcinoembrionário |
| Beneficiário: | Denise Gonçalves Priolli |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |