A comparative study of the modulatory effects of (-)-cubebin on the mutagenicity/recombinogenicity induced by different chemical agents

Autor(es)

Alves de Rezende, Alexandre Azenha ^[1] ; Munari, Carla Carolina ^[2] ; de Oliveira, Pollyanna Francielli ^[2] ; Ferreira, Natalia Helen ^[2] ; Tavares, Denise Crispim ^[2] ; Andrade e Silva, Marcio Luis ^[2] ; Souza Rezende, Karen Cristina ^[2] ; Spano, Mario Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Uberlandia, Inst Genet & Bioquim, Lab Mutagenese, BR-38400902 Uberlandia, MG - Brazil

^[2] Univ Franca, Lab Mutagenesis, Franca, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Chemical Toxicology; v. 55, p. 645-652, MAY 2013.
• Citações Web of Science: 13

Resumo

(-)-Cubebin (CUB) is a lignan isolated from dry seeds of Piper cubeba. We aimed to assess its genotoxic potential and influence on chromosomal damage (frequency of micronuclei - MN) induced by doxorubicin (DXR) in V79 cells and by urethane (URE) in somatic Drosophila melanogaster cells. Our findings indicate an absence of a CUB-mediated genotoxic effect at the concentrations tested. The results also revealed that CUB significantly reduced the frequency of MN induced by DXR, with a mean reduction of 63.88%. In a previous study, our research group demonstrated an absence of CUB-mediated mutagenic effects through the wing Somatic Mutation and Recombination Test (SMART) in Drosophila. In the present study, we used the standard and high bioactivation versions of the SMART to estimate the antigenotoxic effects of CUB associated with URE. At lower concentrations, the recombination level decreased, but at the highest concentration, the recombination level increased. Our data and previous studies suggest that CUB may act as a free radical scavenger at low concentrations, a pro-oxidant at higher concentrations when it interacts with the enzymatic system that catalyzes the metabolic detoxification of DXR or URE, and/or an inducer of recombinational DNA repair. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/18219-3 - Avaliação do potencial genotóxico e antigenotóxico da lignana (-)-cubebina em células V79 pelo teste de micronúcleos
• Beneficiário: Natália Helen Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica