Adjunctive Antimicrobial Photodynamic Treatment of Experimentally Induced Periodontitis in Rats With Ovariectomy

Autor(es)

Garcia, Valdir Gouveia ^{[1, 2]} ; Gualberto Junior, Erivan Clementino ^[1] ; Fernandes, Leandro Araujo ^[3] ; Bosco, Alvaro Francisco ^[1] ; Hitomi Nagata, Maria Jose ^[1] ; Casatti, Claudio Aparecido ^[4] ; Ervolino, Edilson ^[4] ; Theodoro, Leticia Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Surg & Integrated Clin, Grp Res & Study Laser Dent, Div Periodont, Aracatuba, SP - Brazil

^[2] Dent Sch Barretos, Univ Ctr Educ Fdn Barretos, Master Course, Barretos, SP - Brazil

^[3] Univ Fed Alfenas, Dept Clin & Surg, Alfenas, MG - Brazil

^[4] UNESP, Dept Basic Sci, BR-16015050 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 84, n. 4, p. 556-565, APR 2013.
• Citações Web of Science: 23

Resumo

Background: The aim of this study is to compare antimicrobial photodynamic therapy (aPDT) as an adjunctive therapy to scaling and root planing (SRP) for the treatment of experimentally induced periodontitis in rats with ovariectomy (OVX) that are or are not treated with estrogen replacement. Methods: A total of 270 female rats were divided into three groups: 1) normal rats; 2) rats with OVX; and 3) rats with OVX with estrogen replacement. Periodontal disease was induced through the introduction of a cotton thread around the mandibular left first molar. After 7 days, the ligature was removed, and the rats were randomly divided into the following treatment groups: 1) SRP plus saline solution; 2) SRP plus low-level laser therapy (LLLT); and 3) SRP plus toluidine blue O irrigation followed by LLLT. Ten rats from each group were euthanized at days 7, 15, and 30 after dental treatment. Bone loss (BL) in the furcation region was evaluated using histometric and immunohistochemical analyses. Results: aPDT treatment resulted in reduced BL compared with SRP treatment at all time points. Additionally, rats treated with aPDT exhibited reduced numbers of tartrate-resistant acid-phosphatase-positive cells and more proliferating cell nuclear antigen-positive cells in all treatment groups regardless of estrogen status. Whereas rats treated with aPDT showed weak immunoreactivity to the receptor activator of nuclear factor-kappa B ligand at day 7 post-treatment, strong osteoprotegerin immunoreactivity was observed at day 15 post-treatment. Conclusion: aPDT is an effective adjunctive therapy for the treatment of periodontitis in rats with OVX that are or are not given estrogen replacement therapy. J Periodontol 2013; 84:556-565. (AU)

• Processo FAPESP: 08/52707-2 - Efeitos do laser e da terapia fotodinamica no tratamento periodontal de ratas ovariectomizadas, com ou sem reposicao hormonal: estudo histomorfometrico e imunoistoquimico.
• Beneficiário: Erivan Clementino Gualberto Júnior
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/06218-0 - Efeitos do laser e da terapia fotodinâmica no tratamento periodontal de ratas ovariectomizadas, com ou sem reposição hormonal: estudo histomorfométrico e imunoistoquímico
• Beneficiário: Valdir Gouveia Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular