Immunomodulatory effect of the alkaloidic extract of Solanum lycocarpum fruits in mice infected with Schistosoma mansoni

Autor(es):Mostrar menos -

Miranda, Mariza Abreu ^[1] ; Kuehn, Christian Collins ^[1] ; Rito Cardoso, Joao Felipe ^[2] ; Rodrigues Oliveira, Luiz Gustavo ^[1] ; Magalhaes, Lizandra Guidi ^[3] ; Jorge Tiossi, Renata Fabiane ^[1] ; Rodrigues, Vanderlei ^[3] ; Zucolloto, Sergio ^[2] ; Prado, Jr., Jose Clovis ^[1] ; McChesney, James Dewey ^[4] ; Bastos, Jairo Kenupp ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Riberao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Patol & Med Legal, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Arbor Therapeut LLC, Mississippi, MS 38627 - USA

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 133, n. 4, p. 396-402, APR 2013.
• Citações Web of Science: 8

Resumo

Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma; it accounts for more than 280,000 deaths annually. In this work we investigated the effect of the alkaloidic extract obtained by acid-base extraction of the dried fruits of Solanum lycocarpum on schistosomiasis. We used this extract at concentrations of 10, 20, and 40 mg/kg to treat mice infected with Schistosoma mansoni in different phases of the parasite cycle, and we compared its effect with that of the positive control praziquantel (60 mg/kg). We evaluated the results on the basis of the number of macrophages, eggs, and granulomas; we also assessed nitric oxide (NO) and interferon-gamma (IFN-gamma) production. Animals treated with a daily dose of 10 or 20 mg/kg alkaloidic extract between the 37th and 41st day of infection showed increased number of macrophages, elevated NO and IFN-gamma concentrations, and reduced number of eggs and granulomas in the liver. The alkaloidic extract of S. lycocarpum fruits displayed an immunomodulatory effect on mice infected with S. mansoni, so its potential to treat schistosomiasis deserves further studies. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 08/56324-0 - Avaliacao do potencial antiparasitario dos glicoalcaloides dos frutos de solanum lycocarpum.
• Beneficiário: Mariza Abreu Miranda
• Modalidade de apoio: Bolsas no Brasil - Mestrado