Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women

Texto completo
Autor(es):
Morceli, Gliciane [1] ; Honorio-Franca, Adenilda C. [2] ; Fagundes, Danny L. G. [1] ; Calderon, Iracema M. P. [1] ; Franca, Eduardo L. [2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Post Grad Program Gynecol Obstet & Mastol, Sao Paulo - Brazil
[2] Univ Fed Mato Grosso, Inst Biol & Hlth Sci, Barra Do Garcas, Mato Grosso - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 8, n. 2 FEB 20 2013.
Citações Web of Science: 22
Resumo

Melatonin is involved in a number of physiological and oxidative processes, including functional regulation in human milk. The present study investigated the mechanisms of action of melatonin and its effects on the functional activity of colostral phagocytes in diabetic women. Colostrum samples were collected from normoglycemic (N = 38) and diabetic (N = 38) women. We determined melatonin concentration, superoxide release, bactericidal activity and intracellular Ca2+ release by colostral phagocytes treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) and incubated with melatonin and its precursor (N-acetyl-serotonin-NAS), antagonist (luzindole) and agonist (chloromelatonin-CMLT). Melatonin concentration was higher in colostrum samples from hyperglycemic than normoglycemic mothers. Melatonin stimulated superoxide release by colostral phagocytes from normoglycemic but not hyperglycemic women. NAS increased superoxide, irrespective of glycemic status, whereas CMTL increased superoxide only in cells from the normoglycemic group. Phagocytic activity in colostrum increased significantly in the presence of melatonin, NAS and CMLT, irrespective of glycemic status. The bactericidal activity of colostral phagocytes against enterophatogenic Escherichia coli (EPEC) increased in the presence of melatonin or NAS in the normoglycemic group, but not in the hyperglycemic group. Luzindole blocked melatonin action on colostrum phagocytes. Phagocytes from the normoglycemic group treated with melatonin exhibited an increase in intracellular Ca2+ release. Phagocytes treated with TMB-8 (intracellular Ca2+ inhibitor) decreased superoxide, bactericidal activity and intracellular Ca2+ release in both groups. The results obtained suggest an interactive effect of glucose metabolism and melatonin on colostral phagocytes. In colostral phagocytes from normoglycemic mothers, melatonin likely increases the ability of colostrum to protect against EPEC and other infections. In diabetic mothers, because maternal hyperglycemia modifies the functional activity of colostrum phagocytes, melatonin effects are likely limited to anti-inflammatory processes, with low superoxide release and bactericidal activity. (AU)

Processo FAPESP: 09/01188-8 - Atividade funcional de fagócitos do leite de mães hiperglicêmicas leve e diabéticas e sua regulação neuroimunoendocrinológica.
Beneficiário:Glilciane Morceli
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/18045-5 - Imunologia das interações materno-fetais no Diabetes e hiperglicemia leve: transferência passiva de componentes imunoreativos do sangue materno e do colostro para o recém-nascido
Beneficiário:Iracema de Mattos Paranhos Calderon
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/09187-8 - Atividade funcional de fagócitos do leite de mães hiperglicêmicas leve e diabéticas e sua regulação neuroimunoendocrinológica
Beneficiário:Iracema de Mattos Paranhos Calderon
Modalidade de apoio: Auxílio à Pesquisa - Regular