Molecular Analysis of the Freshwater Prawn Macrobrachium olfersii (Decapoda, Palaemonidae) Supports the Existence of a Single Species throughout Its Distribution

Autor(es)

Rossi, Natalia ^[1] ; Mantelatto, Fernando Luis ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Bioecol & Crustacean Systemat LBSC, Program Comparat Biol, Dept Biol, Fac Philosophy Sci & Letters Ribeirao P, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 1 JAN 31 2013.
• Citações Web of Science: 24

Resumo

Macrobrachium olfersii is an amphidromous freshwater prawn, widespread along the eastern coasts of the Americas. This species shows great morphological modifications during ontogenesis, and several studies have verified the existence of a wide intraspecific variation. Because of this condition, the species is often misidentified, and several synonyms have been documented. To elucidate these aspects, individuals of M. olfersii from different populations along its range of distribution were investigated. The taxonomic limit was established, and the degree of genetic variability of this species was described. We extracted DNA from 53 specimens of M. olfersii, M. americanum, M. digueti and M. faustinum, which resulted in 84 new sequences (22 of 16S mtDNA, 45 of Cythocrome Oxidase I (COI) mtDNA, and 17 of Histone (H3) nDNA). Sequences of three genes (single and concatenated) from these species were used in the Maximum Likelihood and Bayesian Inference phylogenetic analyses and COI sequences from M. olfersii were used in population analysis. The genetic variation was evaluated through the alignment of 554 bp from the 16S, 638 bp from the COI, and 338 bp from the H3. The rates of genetic divergence among populations were lower at the intraspecific level. This was confirmed by the haplotype net, which showed a continuous gene flow among populations. Although a wide distribution and high morphological intraspecific variation often suggest the existence of more than one species, genetic similarity of Caribbean and Brazilian populations of M. olfersii supported them as a single species. (AU)

• Processo FAPESP: 09/54931-0 - Melhoria e capacitação das coleções científicas do Departamento de Biologia da Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo
• Beneficiário: Carlos Alberto Garofalo
• Linha de fomento: Auxílio à Pesquisa - Programa Infra-estrutura - Acervos biológicos
• Processo FAPESP: 09/12409-5 - Revisão das espécies de Macrobrachium pertencentes ao "complexo olfersi" (Crustacea, Palaemonidae): análises morfológicas e moleculares
• Beneficiário: Natália Rossi
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/50188-8 - Crustáceos decápodes: multidisciplinaridade na caracterização da biodiversidade marinha do estado de São Paulo (taxonomia, espermiotaxonomia, biologia molecular e dinâmica populacional) (Biodiversidade Marinha)
• Beneficiário: Fernando Luis Medina Mantelatto
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático