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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular Analysis of the Freshwater Prawn Macrobrachium olfersii (Decapoda, Palaemonidae) Supports the Existence of a Single Species throughout Its Distribution

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Author(s):
Rossi, Natalia [1] ; Mantelatto, Fernando Luis [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Lab Bioecol & Crustacean Systemat LBSC, Program Comparat Biol, Dept Biol, Fac Philosophy Sci & Letters Ribeirao P, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLoS One; v. 8, n. 1 JAN 31 2013.
Web of Science Citations: 24
Abstract

Macrobrachium olfersii is an amphidromous freshwater prawn, widespread along the eastern coasts of the Americas. This species shows great morphological modifications during ontogenesis, and several studies have verified the existence of a wide intraspecific variation. Because of this condition, the species is often misidentified, and several synonyms have been documented. To elucidate these aspects, individuals of M. olfersii from different populations along its range of distribution were investigated. The taxonomic limit was established, and the degree of genetic variability of this species was described. We extracted DNA from 53 specimens of M. olfersii, M. americanum, M. digueti and M. faustinum, which resulted in 84 new sequences (22 of 16S mtDNA, 45 of Cythocrome Oxidase I (COI) mtDNA, and 17 of Histone (H3) nDNA). Sequences of three genes (single and concatenated) from these species were used in the Maximum Likelihood and Bayesian Inference phylogenetic analyses and COI sequences from M. olfersii were used in population analysis. The genetic variation was evaluated through the alignment of 554 bp from the 16S, 638 bp from the COI, and 338 bp from the H3. The rates of genetic divergence among populations were lower at the intraspecific level. This was confirmed by the haplotype net, which showed a continuous gene flow among populations. Although a wide distribution and high morphological intraspecific variation often suggest the existence of more than one species, genetic similarity of Caribbean and Brazilian populations of M. olfersii supported them as a single species. (AU)

FAPESP's process: 09/54931-0 - Improvement and management of biological specimen collections at the Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo
Grantee:Carlos Alberto Garofalo
Support type: Research Infrastructure Program - Biological specimen collections
FAPESP's process: 09/12409-5 - Revision of Macrobrachium species belonging to the "olfersi complex" (Crustacea, Palaemonidae): morphological and molecular analysis
Grantee:Natália Rossi
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/50188-8 - Decapod crustaceans: multi-disciplinary characterization of the sea biodiversity of the State of São Paulo (taxonomy, spermiotaxonomy, molecular biology and population dynamics)
Grantee:Fernando Luis Medina Mantelatto
Support type: BIOTA-FAPESP Program - Thematic Grants